Analysis of four circulating complexes of insulin-like growth factor binding proteins in human blood during aging
| Autor: | Vesna Malenković, Nikola Gligorijević, Goran Miljuš, Olgica Nedić, Miloš Šunderić |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Electrophoresis Male 0301 basic medicine Aging medicine.medical_treatment Blotting Western Biophysics Plasma protein binding Ligands Fibrinogen Biochemistry Biochemistry Genetics and Molecular Biology (miscellaneous) Insulin-like growth factor-binding protein Iodine Radioisotopes Protein Carbonylation 03 medical and health sciences 0302 clinical medicine Macroglobulins medicine Humans Receptor Aged Aged 80 and over chemistry.chemical_classification biology Chemistry Growth factor Transferrin Blood Proteins General Medicine Middle Aged Blood proteins Insulin-Like Growth Factor Binding Proteins Blot 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Female Geriatrics and Gerontology hormones hormone substitutes and hormone antagonists Protein Binding medicine.drug |
| Zdroj: | Biochemistry (Moscow). 82:1200-1206 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1134/s0006297917100133 |
| Popis: | The primary role of insulin-like growth factor binding proteins (IGFBPs) is to regulate availability of IGFs for interacting with receptors, but IGFBPs perform IGF-independent actions as well. The availability and activity of IGFBPs in the circulation is influenced primarily by their concentration and structural modifications, but possibly also by interaction with major plasma proteins such as transferrin, alpha-2-macroglobulin (α2M), and fibrinogen. Four types of circulating IGFBP complexes were examined in this study by immuno- and ligand-binding assays in adults of different age. The amounts of IGFBP-3/transferrin and IGFBP-1/fibrinogen complexes were similar in middle- and old-aged persons, whereas the amounts of IGFBP-1 (or -2)/α2M monomer complexes were lower in the old-aged group and negatively correlated with total IGFBP-1 (or -2) amounts in blood. In contrast to IGFBP-1, IGFBP-2 was present in significantly greater quantities in complexes with α2M dimer than α2M monomer in older individuals. IGFBP complexes did not bind 125I-labeled IGF-I in amounts detectable by ligand blotting. According to the results of this study, the quantities of IGFBP-1 and IGFBP-2, which interact with α2M, are age-dependent and, in the case of complexes with α2M monomer, they are negatively correlated with the total circulating levels of these two IGFBPs. |
| Databáze: | OpenAIRE |
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