Characterization of the murine Th2 response to immunization with liposomal M2e influenza vaccine
Autor: | Jill Adler-Moore, Gary Fujii, Hana Kim, Chris Petro, Juan Romero, Gretchen Jimenez, Meilen Munoz, Ernst William A, Terrence M. Tumpey, Suzie Kosina, Hui Zeng |
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Rok vydání: | 2011 |
Předmět: |
medicine.drug_class
Influenza vaccine Molecular Sequence Data Orthomyxoviridae Hemagglutinin (influenza) Antibodies Viral Monoclonal antibody Virus Viral Matrix Proteins Mice Influenza A Virus H1N1 Subtype Th2 Cells Orthomyxoviridae Infections Antigen medicine Animals Humans Amino Acid Sequence Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health biology.organism_classification Virology Lipid A Infectious Diseases Immunization Influenza Vaccines Liposomes biology.protein Cytokines Molecular Medicine Female Antibody |
Zdroj: | Vaccine. 29:4460-4468 |
ISSN: | 0264-410X |
Popis: | While the current influenza vaccine strategy is dependent on eliciting neutralizing antibodies to the hemagglutinin (H or HA) surface glycoprotein, antigenic drifts and occasional antigenic shifts necessitate constant surveillance and annual updates to the vaccine components. The ectodomain of the matrix 2 (M2e) channel protein has been proposed as a universal vaccine candidate, although it has not yet been shown to elicit neutralizing antibodies. Utilizing a liposome-based vaccine technology, an M2e vaccine (L-M2e-HD/MPL) was tested and shown to stimulate the production of anti-M2e antibodies which precipitated with whole virus and inhibited viral cell lysis by multiple type A strains of influenza virus using a novel in vitro assay. The anti-M2e antibodies also conferred complete protection following passive transfer from L-M2e-HD/MPL vaccinated mice to naïve mice challenged with H1N1 virus. Significantly higher levels of IL-4 compared to IFN-γ were secreted by the splenocytes of L-M2e-HD/MPL vaccinated mice incubated with M2e. In addition, depletion of CD4 cells or CD4 cells plus CD8 cells from L-M2e-HD/MPL vaccinated mice using monoclonal antibodies markedly decreased the level of protection of the vaccine when compared to just CD8 depletion of L-M2e-HD/MPL vaccinated mice. These results suggest that the protective immune response elicited by this vaccine is mediated primarily by a Th2 mechanism. |
Databáze: | OpenAIRE |
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