Antiproliferative activity, antioxidant capacity and chemical composition of extracts from the leaves and stem of Chresta sphaerocephala
| Autor: | Marcos José Salvador, Nathalia Luiza Andreazza, Diones Aparecida Dias, João Carvalho, Ildenize B. S. Cunha, Ana Lúcia Tasca Gois Ruiz, Larissa Saito da Costa, Wallace Ribeiro Corrêa, Elisandra Cristina Schinor |
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| Rok vydání: | 2015 |
| Předmět: |
Chresta sphaerocephala
Flavonoids Antiproliferative and antioxidant activities Chromatography Antioxidant biology DPPH Terpenoids medicine.medical_treatment Ethyl acetate lcsh:RS1-441 Asteraceae biology.organism_classification Terpenoid lcsh:Pharmacy and materia medica Hexane Pharmacology Toxicology and Pharmaceutics(all) chemistry.chemical_compound chemistry Biochemistry medicine Steroids General Pharmacology Toxicology and Pharmaceutics Growth inhibition Medicinal plants |
| Zdroj: | Revista Brasileira de Farmacognosia, Volume: 25, Issue: 4, Pages: 369-374, Published: AUG 2015 Revista Brasileira de Farmacognosia v.25 n.4 2015 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA Revista Brasileira de Farmacognosia, Vol 25, Iss 4, Pp 369-374 Revista Brasileira de Farmacognosia, Vol 25, Iss 4, Pp 369-374 (2015) |
| ISSN: | 0102-695X |
| Popis: | In this study, antiproliferative and antioxidant activities of crude extracts (hexane, ethyl acetate and methanol) from leaves and stem of Chresta sphaerocephala DC., Asteraceae, were investigated. Antiproliferative activity was tested in vitro against ten human cancer cells and against VERO (no cancer cell). Antioxidant activities were determined using DPPH and ORAC-FL assays and the total phenolic content was estimated by Folin–Ciocalteu method. Hexane and ethyl acetate extracts (leaves and stem) exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 50.40 and 250 μg/ml. For VERO cell, TGI values were >250 μg/ml for all extracts, except to hexane extract of the stem (TGI 80.92 μg/ml). In an initial evaluation, ethyl acetate and methanol extracts (leaves and stem) have shown levels of phenolic compounds between 6.94 and 30.96 mg GAE/kg in Folin–Ciocalteu assay, DPPH free-radical scavenging activity with SC50 in the range of 75.22 and 400 μg/ml and antioxidant capacity between 290.08 and 1088 μmol TE/g of extract in ORAC-FL assay. HPLC-DAD and ESI-MS analysis allowed the identification of flavonoids in the methanol extract from the leaves of C. sphaerocephala. Three steroids and nine triterpenoids were identified in the bioactive hexane extracts using HRGC. Keywords: Chresta sphaerocephala, Steroids, Terpenoids, Flavonoids, Antiproliferative and antioxidant activities |
| Databáze: | OpenAIRE |
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