A hold-and-feed mechanism drives directional DNA loop extrusion by condensin

Autor:	Indra A. Shaltiel, Sumanjit Datta, Léa Lecomte, Markus Hassler, Marc Kschonsak, Sol Bravo, Catherine Stober, Jenny Ormanns, Sebastian Eustermann, Christian H. Haering
Rok vydání:	2022
Předmět:	Adenosine Triphosphatases DNA-Binding Proteins Multidisciplinary Multiprotein Complexes Cryoelectron Microscopy Nucleic Acid Conformation DNA Single Molecule Imaging
Zdroj:	Science (New York, N.Y.). 376(6597)
ISSN:	1095-9203
Popis:	Structural maintenance of chromosomes (SMC) protein complexes structure genomes by extruding DNA loops, but the molecular mechanism that underlies their activity has remained unknown. We show that the active condensin complex entraps the bases of a DNA loop transiently in two separate chambers. Single-molecule imaging and cryo–electron microscopy suggest a putative power-stroke movement at the first chamber that feeds DNA into the SMC–kleisin ring upon adenosine triphosphate binding, whereas the second chamber holds on upstream of the same DNA double helix. Unlocking the strict separation of “motor” and “anchor” chambers turns condensin from a one-sided into a bidirectional DNA loop extruder. We conclude that the orientation of two topologically bound DNA segments during the SMC reaction cycle determines the directionality of DNA loop extrusion.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b31f6cb5b5964276abbe4a261b2ec381 https://pubmed.ncbi.nlm.nih.gov/35653469 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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