Plasmalogen phospholipids are involved in HDL-mediated cholesterol efflux: insights from investigations with plasmalogen-deficient cells
| Autor: | Rakefet Sharf, Michael Aviram, Hanna Mandel, Ronald J.A. Wanders, Moshe Berant, Peter Vreken |
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| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Plasmalogen Plasmalogens Mutant Biophysics Phospholipid Biology Biochemistry Cell Line Mice chemistry.chemical_compound Internal medicine medicine Animals Humans Molecular Biology Phospholipids Rhizomelic chondrodysplasia punctata Cholesterol Macrophages Biological Transport Cell Biology medicine.disease Endocrinology chemistry Cell culture lipids (amino acids peptides and proteins) Efflux Lipoproteins HDL Lipoprotein |
| Zdroj: | Biochemical and biophysical research communications, 250(2), 369-373. Academic Press Inc. |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.9321 |
| Popis: | Plasmalogens are ether-glycerophospholipids that exist in all mammalian cells, but their physiological function remains thus far an enigma. It has been previously suggested that the association of high-density lipoprotein (HDL) with cellular phospholipid is a pre-requisite for the process of HDL-mediated cholesterol efflux (HDL-MCE). To investigate our hypothesis that plasmalogens might play a role in HDL-MCE, we used a model composed of plasmalogen-deficient cells including RAW mutant macrophages and fibroblasts from patients with rhizomelic chondrodysplasia punctata type II. In mutant macrophages, HDL-MCE was reduced by 57% compared to control macrophages, after 16 hours. A similar phenomenon was observed in plasmalogen-deficient patients fibroblasts. Incubation of plasmalogen-deficient fibroblasts with 1-0-hexadecyl-sn-glycerol, which restored plasmalogen levels to that of control cells, resulted in a 35% increase in HDL-MCE, compared to a 10% increment in controls. The novel finding that HDL-MCE is reduced in plasmalogen-deficient cells and increases following plasmalogen restoration leads us to suggest that plasmalogen has an important function in the mediation of cellular cholesterol efflux. |
| Databáze: | OpenAIRE |
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