Circular RNA hsa_circ_0043278 inhibits breast cancer progression via the miR-455-3p/EI24 signalling pathway
Autor: | Chong Liu, Yue Shi |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Adult
Cancer Research Cell Survival Down-Regulation Mice Nude Breast Neoplasms Small hairpin RNA Mice Breast cancer Downregulation and upregulation Cell Line Tumor Genetics Animals Humans Genes Tumor Suppressor Neoplasm Invasiveness Circulating MicroRNA Circular RNA RC254-282 Aged Cell Proliferation Aged 80 and over Reporter gene Mice Inbred BALB C Cell growth Chemistry Research Nuclear Proteins Neoplasms. Tumors. Oncology. Including cancer and carcinogens Cell migration Transfection RNA Circular Middle Aged hsa_circ_0043278 Molecular biology In vitro MicroRNAs Oncology Cell culture Disease Progression MCF-7 Cells Heterografts Female Apoptosis Regulatory Proteins Neoplasm Transplantation |
Zdroj: | BMC Cancer, Vol 21, Iss 1, Pp 1-14 (2021) BMC Cancer |
ISSN: | 1471-2407 |
Popis: | Background Breast cancer (BC) is one of the major malignancies worldwide. Circular ribonucleic acids (circRNAs) are a class of conserved ribonucleic acid (RNA) molecules that play important roles in various diseases. Recently, circRNAs have been suggested to have diagnostic value and may function as potential diagnostic biomarkers for BC. Previously, hsa_circ_0043278 was found to be downregulated in human BC. However, its role in human BC has not yet been identified. Methods The levels of hsa_circ_0043278 in BC cell lines were verified by quantitative reverse transcription–polymerase chain reaction (qRT–PCR). The overexpression vector and short hairpin RNA (shRNA) of hsa_circ_0043278 were transfected into MDA-MB-231 and MCF-7 cells, respectively. The effects of hsa_circ_0043278 on tumour cell growth, migration and invasion were measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), colony formation, wound healing and Transwell assays in vitro. A xenograft experiment was conducted to validate the inhibitory effect of hsa_circ_0043278 on tumour growth. The interaction between hsa_circ_0043278 and miR-455-3p was confirmed by a dual-luciferase reporter assay. Mimics and inhibitors of miR-455-3p were designed to confirm the influence of hsa_circ_0043278 on the hsa_circ_0043278/miR-455-3p/etoposide-induced gene 24 (EI24) axis. Results Hsa_circ_0043278 was downregulated in BC cell lines. Furthermore, overexpression of hsa_circ_0043278 notably decreased BC cell viability and inhibited BC cell migration and invasion in vitro and suppressed tumour growth in vivo. Downregulation of hsa_circ_0043278 led to the opposite results. Hsa_circ_0043278 expression was negatively correlated with that of miR-455-3p. In addition, mechanistic investigation proved that hsa_circ_0043278 directly bound to miR-455-3p and regulated EI24 and NF-κB expression in BC cells. Conclusion Hsa_circ_0043278 acts as a tumour suppressor gene in BC through the hsa_circ_0043278/miR-455-3p/EI24 axis and may be regarded as a new prognostic predictor or potential therapeutic target in BC. |
Databáze: | OpenAIRE |
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