Cosmetics Europe multi-laboratory pre-validation of the SkinEthic™ reconstituted human corneal epithelium test method for the prediction of eye irritation
| Autor: | Monique Marrec-Fairley, Nathalie Alépée, A. De Smedt, M. Tailhardat, Pauline McNamee, Sandrine Bessou-Touya, B. Le Varlet, Claudine Faller, B. De Wever, Uwe Pfannenbecker, José Cotovio, F. Van Goethem, Penny Jones |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
media_common.quotation_subject Cosmetics In Vitro Techniques Toxicology medicine.disease_cause Animal Testing Alternatives Technology Transfer Ophthalmology Toxicity Tests medicine Humans Pre validation media_common Corneal epithelium Reproducibility business.industry Epithelium Corneal Reproducibility of Results Eye irritation General Medicine Test method Europe medicine.anatomical_structure Technology transfer Irritants Irritation business Laboratories |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 27(5) |
| ISSN: | 1879-3177 |
| Popis: | Cosmetics Europe, The Personal Care Association, known as Colipa before 2012, conducted a program of technology transfer and assessment of Within/Between Laboratory (WLV/BLV) reproducibility of the SkinEthic™ Reconstituted Human Corneal Epithelium (HCE) as one of two human reconstructed tissue eye irritation test methods. The SkinEthic™ HCE test method involves two exposure time treatment procedures – one for short time exposure (10 min – SE) and the other for long time exposure (60 min – LE) of tissues to test substance. This paper describes pre-validation studies of the SkinEthic™ HCE test method (SE and LE protocols) as well as the Eye Peptide Reactivity Assay (EPRA). In the SE WLV study, 30 substances were evaluated. A consistent outcome with respect to viability measurement across all runs was observed with all substances showing an SD of less than 18%. In the LE WLV study, 44 out of 45 substances were consistently classified. These data demonstrated a high level of reproducibility within laboratory for both the SE and LE treatment procedures. For the LE BLV, 19 out of 20 substances were consistently classified between the three laboratories, again demonstrating a high level of reproducibility between laboratories. The results for EPRA WLV and BLV studies demonstrated that all substances analysed were categorised similarly and that the method is reproducible. The SkinEthic™ HCE test method entered into the experimental phase of a formal ECVAM validation program in 2010. |
| Databáze: | OpenAIRE |
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