Comparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma
| Autor: | Jean-François Beckers, Joseph Sulon, Z. Perenyi, Ottó Szenci, Pierre Drion |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Quality Control
medicine.medical_specialty medicine.medical_treatment Radioimmunoassay Gestational Age Pregnancy Proteins Endocrinology Pregnancy Reference Values Placenta Internal medicine Blood plasma medicine Animals Aspartic Acid Endopeptidases Insemination Artificial chemistry.chemical_classification Antiserum Artificial insemination medicine.disease medicine.anatomical_structure nervous system chemistry Gestation Animal Science and Zoology Cattle Female Glycoprotein hormones hormone substitutes and hormone antagonists Biotechnology |
| Zdroj: | Reproduction in domestic animals = Zuchthygiene. 37(2) |
| ISSN: | 0936-6768 |
| Popis: | Pregnancy-associated glycoproteins (PAGs) constitute a large family of glycoproteins that are synthesized in the superficial layer of the ruminant placenta according to a spatial and temporal expression pattern. When PAGs are released in the maternal blood they can be used for pregnancy diagnosis, pregnancy follow-up and for the monitoring of the trophoblastic function. Three different radioimmunoassay systems (RIA 1, RIA 2 and RIA 3) using antisera produced against PAG I67 (RIA 1), PAG55+62 (RIA 2) and PAG55+59 (RIA 3) were used in this investigation in order to measure the PAG concentration in plasma samples withdrawn from pregnant cows and heifers during different periods following artificial insemination (AI). These systems were able to detect PAG molecules in the maternal blood as early as 21 days after AI in different concentrations (RIA 1: 0.43 +/- 0.24 ng/ml, mean +/- SD; RIA 2: 0.48 +/- 0.24 ng/ml; RIA 3: 0.64 +/- 0.37 ng/ml). On days 32 and 42 RIA 2 (4.30 +/- 1.32 ng/ml and 5.56 +/- 1.95 ng/ml) and RIA 3 (4.17 +/- 1.15 ng/ml and 5.60 +/- 1.89 ng/ml) presented significantly (p0.0001) higher PAG concentrations than those of RIA 1 (2.43 +/- 0.81 ng/ml and 4.01 +/- 1.48 ng/ml), respectively. After day 21, significant correlations (p0.0001; r/= 0.929) were determined between the three systems. Additionally the three individual PAG profiles presented in this study showed that PAG molecules secreted in the maternal blood between 21 and 50 days after AI were better recognized by the RIA 2 and RIA 3 systems. This study clearly indicated that the ability of a RIA test to recognize PAG molecules in the maternal blood can be improved by carefully selecting the antiserum. |
| Databáze: | OpenAIRE |
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