Electrical Cartridge Sensor Enables Reliable and Direct Identification of MicroRNAs in Urine of Patients
| Autor: | Kwan Hyi Lee, Seongchan Kim, Sungwook Park, Seok Ho Kang, Young Hoon Kim, Young Soo Cho, Jong Hyun Tae, Tae Il No, Ji Sung Shim, Youngdo Jeong |
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| Rok vydání: | 2020 |
| Předmět: |
Peptide Nucleic Acids
Bioengineering 02 engineering and technology Urine 01 natural sciences Electricity microRNA Medicine Humans Nanotechnology In patient Disposable Equipment Instrumentation Volume concentration Fluid Flow and Transfer Processes business.industry Process Chemistry and Technology 010401 analytical chemistry Urological Diseases 021001 nanoscience & nanotechnology 0104 chemical sciences MicroRNAs Clinical diagnosis Graphite 0210 nano-technology business Signal amplification Biomedical engineering |
| Zdroj: | ACS sensors. 6(3) |
| ISSN: | 2379-3694 |
| Popis: | Urinary miRNAs are biomarkers that demonstrate considerable promise for the noninvasive diagnosis and prognosis of diseases. However, because of background noise resulting from complex physiological features of urine, instability of miRNAs, and their low concentration, accurate monitoring of miRNAs in urine is challenging. To address these limitations, we developed a urine-based disposable and switchable electrical sensor that enables reliable and direct identification of miRNAs in patient urine. The proposed sensing platform combining disposable sensor chips composed of a reduced graphene oxide nanosheet and peptide nucleic acid facilitates the label-free detection of urinary miRNAs with high specificity and sensitivity. Using real-time detection of miRNAs in patient urine without pretreatment or signal amplification, this sensor allows rapid, direct detection of target miRNAs in a broad dynamic range with a detection limit down to 10 fM in human urine specimens within 20 min and enables simultaneous quantification of multiple miRNAs. As confirmed using a blind comparison with the results of pathological examination of patients with prostate cancer, the sensor offers the potential to improve the accuracy of early diagnosis before a biopsy is taken. This study holds the usefulness of the practical sensor for the clinical diagnosis of urological diseases. |
| Databáze: | OpenAIRE |
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