Chikungunya Virus Infects the Heart and Induces Heart-Specific Transcriptional Changes in an Immunodeficient Mouse Model of Infection
| Autor: | Andrew Routh, Richard J. Holcomb, Rose M. Langsjoen, Yiyang Zhou |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Mice
Nude Alphavirus Viral Nonstructural Proteins Biology Virus Replication medicine.disease_cause Article Virus Mice Virology medicine Animals Chikungunya Pathogen Gene Cardiac muscle virus diseases Skeletal muscle Heart biology.organism_classification Disease Models Animal Infectious Diseases medicine.anatomical_structure Togaviridae Chikungunya Fever Parasitology Chikungunya virus |
| Zdroj: | Am J Trop Med Hyg |
| ISSN: | 1476-1645 0002-9637 |
| Popis: | Chikungunya virus (CHIKV) is a mosquito-transmitted pathogen in family Togaviridae, genus Alphavirus. Although CHIKV is well known for its ability to cause debilitating rheumatoid-like arthritis, it has been also been observed to cause cardiovascular symptoms such as arrhythmias. Here, using samples from a previous study, we sequenced RNA from serum, kidney, skeletal muscle, and cardiac muscle from CHIKV- and mock-infected IFN-αR−/− mice using two sequencing techniques to investigate heart-specific changes in virus mutational profiles and host gene expression. Mutation rates were similar across muscle tissues although heart tissue carried heart-specific CHIKV minority variants, one of which had a coding change in the nsP3 gene and another in the 3′UTR. Importantly, heart-specific transcriptional changes included differential expression of genes critical for ion transport and muscle contraction. These results demonstrate that CHIKV replicates in the hearts of immunodeficient mice and induce heart-specific mutations and host responses with implications for cardiac pathologies. |
| Databáze: | OpenAIRE |
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