Using enzymatic amplification by aldolase for the optical detection of DNA by an artificial signal cascade
| Autor: | Roland Krämer, Susanne Kassube, Nora Graf |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Optics and Photonics
Cations Divalent Pyridines Clinical Biochemistry DNA Single-Stranded Pharmaceutical Science Fructose-bisphosphate aldolase Saccharomyces cerevisiae Biochemistry Catalysis Cofactor chemistry.chemical_compound Fructose-Bisphosphate Aldolase Drug Discovery DNA Fungal Molecular Biology chemistry.chemical_classification biology Chemistry Organic Chemistry Aldolase A Nucleic acid sequence Chromophore Enzyme Activation Zinc Enzyme Cascade biology.protein Molecular Medicine DNA Signal Transduction |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 18:4786-4788 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2008.07.102 |
| Popis: | A two-step reaction cascade is applied to the sequence-specific detection of single-stranded DNA, including analyte-triggered re-activation of apo-aldolase by its cofactor Zn2+ and catalytic conversion of a chromophore. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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