The Expression and Function of the NKRP1 Receptor Family in C57BL/6 Mice
| Autor: | Ella Buchanan, Katarzyna M. Mickiewicz, Frances Gays, Colin G. Brooks, Jonathan G. Aust |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cytotoxicity
Immunologic Immunology B-Lymphocyte Subsets Major histocompatibility complex NKG2 Binding Competitive Epitopes Mice Interleukin 21 T-Lymphocyte Subsets Cell Line Tumor medicine Animals Humans Immunology and Allergy Receptor Cells Cultured Mice Knockout Mice Inbred BALB C biology Antibodies Monoclonal Transfection Natural killer T cell Molecular biology Rats Mice Inbred C57BL medicine.anatomical_structure Gene Expression Regulation Multigene Family Interleukin 12 biology.protein Binding Sites Antibody Bone marrow NK Cell Lectin-Like Receptor Subfamily A |
| Zdroj: | The Journal of Immunology. 183:106-116 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.0804281 |
| Popis: | NKRP1 receptors were discovered more than 20 years ago, but due to a lack of appropriate reagents, our understanding of them has remained limited. Using a novel panel of mAbs that specifically recognize mouse NKRP1A, D, and F molecules, we report here that NKRP1D expression is limited to a subpopulation of NK cells, but in contrast to Ly49 receptors appears to be expressed in a normal codominant manner. NKRP1D− and NKRP1D+ NK cells are functionally distinct, NKRP1D+ cells showing reduced expression of various Ly49 receptors, elevated expression of CD94/NKG2 receptors, and higher IFN-γ secretion and cytotoxicity than NKRP1D− cells. Furthermore, NKRP1D+ NK cells were unable to kill transfected cells expressing high levels of Clr-b molecules, but readily killed MHC class-I-deficient blast cells that express only low levels of Clr-b. NKRP1A and NKRP1F were expressed at low levels on all splenic and bone marrow NK cells, but mAb-induced cross-linking of NKRP1A and NKRP1F caused no significant enhancement or inhibition of NK cell cytotoxicity and no detectable production of IFN-γ. NKRP1A, D, and F expression could not be detected on NKT cells, all of which express NKRP1C, and although some activated T cells expressed NKRP1C and perhaps low levels of NKRP1A, no significant expression of NKRP1D or F could be detected. NKRP1 molecules expressed on NK cells or transfectants were down-regulated by cross-linking with mAbs or cell surface ligands, and using this phenomenon as a functional assay for NKRP1-ligand interaction revealed that NKRP1F can recognize CLR-x. |
| Databáze: | OpenAIRE |
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