The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
| Autor: | Yu Wang, Jiang-Lin Wu, Francisco Javier Sanchez-Garcia, Lang Fu, Ting Qiao, Bao-Zhen Tang, Youming Hou, Zhi-Ming Chen, E Meng |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sequence analysis lcsh:QR1-502 01 natural sciences lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound Complete sequence negative-sense (–) single-stranded RNA virus Virology RNA polymerase Insect virus Peptide sequence Polymerase Genetics biology phylogenetic analysis viral genome RNA RNA virus insect virus biology.organism_classification 010602 entomology 030104 developmental biology Infectious Diseases chemistry dimarhabdovirus biology.protein |
| Zdroj: | Viruses, Vol 11, Iss 3, p 257 (2019) Viruses Volume 11 Issue 3 |
| ISSN: | 1999-4915 |
| Popis: | The complete sequence of a novel RNA virus isolated from Tetrastichus brontispae (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins, hypothetical proteins, matrix proteins, glycoproteins, and RNA-dependent RNA polymerases. Sequence analysis indicated that the RNA-dependent RNA polymerase of TbRV-1 shares a 65% nucleotide and 67% amino acid sequence identity with Hubei dimarhabdovirus 2, suggesting that TbRV-1 is a member of the dimarhabdovirus supergroup. This corresponded to the result of the phylogenetic analysis. The affiliation of TbRV-1 with members of the family Rhabdoviridae was further validated by similar transcription termination motifs (GGAACUUUUUUU) to the Drosophila sigmavirus. The prevalence of TbRV-1 in all tissues suggested that the virus was constitutive of, and not specific to, any wasp tissue. To our knowledge, this is the first report on the complete genome sequence of a dimarhabdovirus in parasitoids. |
| Databáze: | OpenAIRE |
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