A Rapid PCR-Based DNA Test for Enterotoxic Bacillus cereus
| Autor: | Vesa Mäntynen, Kristina Lindström |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Bacillus cereus Enterotoxin Polymerase Chain Reaction Applied Microbiology and Biotechnology law.invention Microbiology Enterotoxins Hemolysin Proteins 03 medical and health sciences Bacterial Proteins law RNA Ribosomal 16S Polymerase chain reaction 030304 developmental biology 0303 health sciences Ecology biology 030306 microbiology Hemolysin biology.organism_classification 16S ribosomal RNA Molecular biology 3. Good health Cereus Food Microbiology Restriction fragment length polymorphism Primer (molecular biology) Polymorphism Restriction Fragment Length Food Science Biotechnology |
| Zdroj: | University of Helsinki |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.64.5.1634-1639.1998 |
| Popis: | The occurrence of DNA sequences encoding the hemolysin HblA complex and Bacillus cereus enterotoxin BceT, which have recently been confirmed as enterotoxins, was studied in Bacillus spp. To amplify these DNA sequences, PCR primer systems for the B component of hblA and for bceT DNA sequences were developed. The results from the amplification of hblA sequences correlated well with results obtained with the B. cereus enterotoxin (diarrheal type) test kit (RPLA kit), but not with the results of the Bacillus diarrheal enterotoxin visual immunoassay (BDE kit). Except for two thermophilic strains, all strains that were positive in PCR amplification assays with the hblA primers were also positive when tested with the RPLA kit. The hblA DNA sequence was found in 33 strains, and these strains were closely related according to 16S rDNA-RFLP analysis, except B. pasteurii . In PCR amplifications with the bceT primers only the model strain gave a positive signal. It is concluded that screening of the hemolysin HblA complex by the PCR method allows faster detection of enterotoxin production than does testing with the RPLA enterotoxin kit. |
| Databáze: | OpenAIRE |
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