Immunohistochemical demonstration of the asialoglycoprotein receptor in rat liver by a sensitive avidin-biotin technique
| Autor: | Jozsef Sipos, Ian G. McFarlane, B.M. McFarlane, C.D. Gove, Roger Williams, C.G. McSorley |
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| Rok vydání: | 1989 |
| Předmět: |
Biotin
Asialoglycoprotein Receptor Antibodies Pathology and Forensic Medicine Immunoenzyme Techniques chemistry.chemical_compound Animals Hepatic Asialoglycoprotein Receptor Receptors Immunologic Antiserum biology Rats Inbred Strains Radioimmunoassay Avidin Molecular biology Rats Liver chemistry Biochemistry Polyclonal antibodies biology.protein Immunohistochemistry Indicators and Reagents Asialoglycoprotein receptor |
| Zdroj: | The Journal of Pathology. 158:247-252 |
| ISSN: | 1096-9896 0022-3417 |
| DOI: | 10.1002/path.1711580313 |
| Popis: | An immunohistochemical technique for the detection of the hepatic asialoglycoprotein receptor (ASGP-R) in cryostat sections of liver by polyclonal and monoclonal anti-ASGP-R antibodies is described. The procedure is based on the alkaline-phosphatase-avidin-biotin complex (ABC-AP) system and important features include fixation of the sections with periodate-lysine-paraformaldehyde (with or without dichromate) and an absolute requirement for blocking of endogenous biotin activity. The sensitivity of the technique is such that binding to ASGP-R can be detected with femtomolar concentrations of monoclonal anti-ASGP-R antibodies and, with polyclonal antisera, approaches that of a radioimmunoassay. |
| Databáze: | OpenAIRE |
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