MOLECULAR GENETIC IDENTIFICATION OF SCOTS PINE AND SIBERIAN LARCH POPULATIONS IN PERM KRAI BASED ON POLYMORPHISM OF ISSR-PCR MARKERS
| Autor: | N Pystogova, Ya. Sboeva, Yu. Vasileva, N Martynenko, Ruslan Kalendar, Nikita Chertov, S. V. Boronnikova |
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| Rok vydání: | 2020 |
| Předmět: |
Genetics
010504 meteorology & atmospheric sciences biology issr-analysis Scots pine dna polymorphism Forestry genetic diversity SD1-669.5 15. Life on land barcodes biology.organism_classification 01 natural sciences 03 medical and health sciences 0302 clinical medicine Polymorphism (computer science) larix sibirica ledeb Identification (biology) 030212 general & internal medicine Larch Issr pcr genetic formulas pinus sylvestris l 0105 earth and related environmental sciences |
| Zdroj: | Сибирский лесной журнал, Vol 7, Iss 4, Pp 35-44 (2020) |
| ISSN: | 2311-1410 |
| DOI: | 10.15372/sjfs20200405 |
| Popis: | The use of DNA-fingerprinting of forest-forming woody plants is considered the most promising tool for genetic control of wood’s geographic origin, the formation of a reliable management system for harvesting and turnover of lumber. The purpose of this work was to search for identification markers, genotyping trees, and molecular genetic identification of previously not studied 2 populations of the Siberian larch Larix sibirica Ledeb. and 4 populations of Scots pine Pinus sylvestris L. of different regions of the Perm Krai. To DNA extraction, from each plant specimens wood were individually obtained and a modified method of extracting DNA from wood was used. In total, the analysis used the DNA of 114 Scots pine trees and 55 Siberian larch trees. For genetic testing, we used ISSR (Inter Simple Sequence Repeats)-method of DNA polymorphism analysis. Genetic identification was performed based on the original author’s method proposed by S. V. Boronnikova and I. V. Boboshina (2014). As a result of molecular genetic analysis, 74 ISSR markers were found and analyzed in populations of Scots pine, 85 ISSR markers were identified in populations of the Siberian larch, and the share of polymorphic loci in both species was high. As a result of molecular genetic identification, identification of species and polymorphic ISSR-markers and their combinations were found that characterize the belonging of trees to a species, as well as to a specific population. Molecular genetic formulas and barcodes for each individual population of two species have been compiled. The effectiveness, stability, and reproducibility of detected identification markers and their combinations have been proven through anonymous testing. The data obtained are the basis for determining the place of origin of wood, which will allow us to recommend measures to counter illegal logging and reduce the damage to the budget of logging regions of Russia, such as the Perm Krai. |
| Databáze: | OpenAIRE |
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