Adult derived mononuclear bone marrow cells improve survival in a model of acetaminophen-induced acute liver failure in rats
| Autor: | Marcos Eugênio Soares Duarte, Maria Cristina Ramos Belardinelli, Roberto Giugliani, Themis Reverbel da Silveira, Carlos Oscar Kieling, Guilherme Baldo, Ursula da Silveira Matte, Luise Meurer, Fernanda dos Santos Pereira, Ângela Maria Vicente Tavares |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Pathology Necrosis medicine.medical_treatment Intraperitoneal injection Toxicology Peripheral blood mononuclear cell Internal medicine Medicine Animals Ficoll Rats Wistar Survival rate Acetaminophen Bone Marrow Transplantation business.industry digestive oral and skin physiology Analgesics Non-Narcotic Liver Failure Acute Rats Survival Rate Disease Models Animal Endocrinology medicine.anatomical_structure Liver Female Bone marrow medicine.symptom Stem cell business Adult stem cell medicine.drug |
| Zdroj: | Toxicology. 247(1) |
| ISSN: | 0300-483X |
| Popis: | Introduction Acute liver failure (ALF) is characterized by a rapid loss of hepatic function, with high mortality. Acetaminophen (APAP) intoxication and viral hepatitis are common causes of ALF. Several studies have shown the capacity of adult bone marrow cells to differentiate in hepatocytes, suggesting their use for treating ALF. Aim In the present study, we tested the use of adult derived mononuclear bone marrow fraction to improve the survival of Wistar rats with APAP-induced ALF. Methods Forty-eight female Wistar rats pre-induced with phenobarbital were given APAP in a single dose of 1 g/kg via intraperitoneal injection. Bone marrow mononuclear cells were purified from male rats using FICOLL gradient and injected through the portal vein in a volume of 0.2 mL containing 1 × 10 6 cells stained with DAPI. Treatment was administered 24 h after APAP injection. The sham group ( n = 24), received 0.2 mL of saline through the portal vein 24 h after APAP administration. Survival, liver histology and ALT levels were observed. Results Survival 72 h post-APAP administration was 33% in the sham group and 70.8% in the group receiving bone marrow cells. Liver histology in treated animals showed less intense necrosis and the presence of DAPI-positive cells. Conclusions We have shown that bone marrow derived cells are capable of significantly increasing the survival rate of APAP-induced ALF in 37.5% (95% CI, 27.8–40.3%). |
| Databáze: | OpenAIRE |
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