Suppression of LPS-induced inflammatory responses by gossypol in RAW 264.7 cells and mouse models
| Autor: | Jingyuan Wei, Lanan Wassy Soromou, Lingxin Duan, Lanxiang Jiang, Xuming Deng, Ruijie Gao, Shuang Guan, Xiurui Cui, Meixia Huo, Haihua Feng, Gefu Chi |
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| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide MAP Kinase Signaling System p38 mitogen-activated protein kinases Immunology Inflammation Biology Pharmacology Lung injury Cell Line Mice chemistry.chemical_compound In vivo medicine Animals Humans Immunology and Allergy Lung RAW 264.7 Cells Gossypium Mice Inbred BALB C Gossypol Lung Injury Disease Models Animal IκBα chemistry Biochemistry Cytokines Inflammation Mediators medicine.symptom |
| Zdroj: | International Immunopharmacology. 15:442-449 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2013.01.008 |
| Popis: | Gossypol, a yellowish polyphenolic compound originally from cotton plant, has been known to exert a potential for anti-cancer, anti-inflammatory and other important therapeutic activities. The purpose of this investigation was to determine the protection of gossypol on inflammation in Lipopolysaccharide (LPS) stimulated RAW 264.7 cells and LPS induced in vivo lung injury model. The effects of gossypol on pro-inflammatory cytokines and signaling pathways were evaluated by enzyme-linked immunosorbent assay and Western blot. The results showed that gossypol significantly inhibited the production of LPS-induced TNF-α, IL-6 and IL-1β both in vitro and vivo. Furthermore, gossypol blocked the phosphorylation of IκBα protein, p65, p38, c-Junterminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in LPS stimulated RAW 264.7 cells. From the in vivo study, it was observed that gossypol attenuated lung histopathologic changes in mouse models. The present data suggest that gossypol suppresses the inflammation in vitro and vivo, and may be a potential therapeutic candidate for the treatment of inflammatory disorders. |
| Databáze: | OpenAIRE |
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