Endothelial fibrinolytic response onto an evolving matrix of fibrin

Autor: Omar Castillo, Rita Marchi, Eduardo Anglés-Cano, Héctor Rojas, Zury Domínguez
Přispěvatelé: Centro de Medicina Experimental, Laboratorio Biología del Desarrollo de la Hemostasia, Instituto Venezolano de Investigaciones Científicas - IVIC - Instituto Venezolano de Investigaciones Científicas - IVIC, Escuela de Bioanálisis (Sede Aragua), Universidad de Carabobo, Instituto de Inmunología, Universidad Central de Venezuela, Laboratorio de Fisiología Celular, Instituto Venezolano de Investigaciones Científicas - IVIC, Instituto de Medicina Experimental, Innovations thérapeutiques en hémostase (IThEM - U1140), Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM), Dr. Eduardo Anglés-Cano is supported by Inserm.This work was partially financed by Oficina de Planificación del SectorUniversitario (OPSU)., Inserm UMR_S 1140 & IVIC (Venezuelz), Instituto Venezolano de Investigaciones Cientificas (IVIC)-Instituto Venezolano de Investigaciones Cientificas (IVIC), Universidad Central de Venezuela (UCV), Instituto Venezolano de Investigaciones Cientificas (IVIC), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Angles-Cano, Eduardo
Předmět:
Zdroj: ResearcherID
BMC Hematology
BMC Hematology, BioMed Central, 2016, 16, 〈10.1186/s12878-016-0048-6〉
BMC Hematology, BioMed Central, 2016, 16, ⟨10.1186/s12878-016-0048-6⟩
ISSN: 2052-1839
DOI: 10.1186/s12878-016-0048-6〉
Popis: International audience; Background: Fibrin provides a temporary matrix at the site of vascular injury. The aims of the present work were (1) to follow fibrin formation and lysis onto the surface of human dermal microvascular endothelial cells (HMEC-1), and (2) to quantify the secretion of fibrinolytic components in the presence of fibrin. Methods: Fibrin clots at different fibrinogen concentrations were formed on top of (model 1) or beneath (model 2) the endothelial cells. Fibrin formation or lysis onto the surface of HMEC-1 cells, was followed by turbidity. Clot structure was visualized by laser scanning confocal microscopy (LSCM). The secretion of uPA and PAI-1 by HMEC-1 cells was quantified by ELISA. Results: The rate of fibrin formation increased approximately 1.5-fold at low fibrinogen content (0.5 and 1 mg/mL; p < 0.05) compared to the condition without cells; however, it was decreased at 2 mg/mL fibrinogen (p < 0.05) and no differences were found at higher fibrinogen concentrations (3 and 5 mg/mL). HMEC-1 retarded dissolution of clots formed onto their surface at 0.5 to 3 mg/mL fibrinogen (p < 0.05). Secretion of uPA was 13 × 10 −6 ng/mL per cell in the absence of RGD and 8 × 10 −6 ng/mL per cell in the presence of RGD, when clots were formed on the top of HMEC-1. However, the opposite was found when cells were grown over fibrin: 6 × 10 −6 ng/mL per cell without RGD vs. 17 × 10 −6 ng/mL per cell with RGD. The secretion of PAI-1 by HMEC-1 cells was unrelated to the presence of fibrin or RGD, 7 × 10 −6 μg/mL per cell and 5 × 10 −6 μg/mL per cell, for the apical (model 1) and basal clots (model 2), respectively. Conclusions: HMEC-1 cells influence fibrin formation and dissolution as a function of the fibrin content of clots. Clot degradation was accentuated at high fibrin concentrations. The secretion of fibrinolytic components by HMEC-1 cells seemed to be modulated by integrins that bind RGD ligands.
Databáze: OpenAIRE
Externí odkaz: