Atractylodes macrocephala polysaccharides induces mitochondrial-mediated apoptosis in glioma C6 cells
Autor: | Xuejun Li, Xianrui Yuan, Chunhai Huang, Fei Liu, Ningrong Ye, Zhao Li |
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Rok vydání: | 2013 |
Předmět: |
Poly ADP ribose polymerase
Apoptosis DNA Fragmentation Biology Biochemistry Structural Biology Polysaccharides Glioma Cell Line Tumor medicine Animals Molecular Biology Polymerase Cell Proliferation Membrane Potential Mitochondrial Caspase 3 Cytochrome c Cytochromes c General Medicine Atractylodes medicine.disease In vitro Caspase 9 Cell biology Mitochondria Rats Cytosol biology.protein DNA fragmentation Poly(ADP-ribose) Polymerases |
Zdroj: | International journal of biological macromolecules. 66 |
ISSN: | 1879-0003 |
Popis: | In recent years there has been an increasing interest in naturally occurring substances in plant origin that may be used as potential chemopreventive and chemotherapeutic agents to prevent or slow the progression of chronic illnesses, such as cancer. In this study, we aimed at examining the antitumor activity of Atractylodes macrocephala polysaccharide (AMPs) in vitro using glioma C6 cells and the underlying mechanisms were also investigated. The results demonstrated that AMPs significantly inhibited proliferation of C6 cells in a concentration dependent manner by DNA fragmentation and apoptosis induction. Besides, AMPs treatment induced the loss of mitochondrial membrane potential and caused release of cytochrome c to cytosol. Furthermore, the activation of capase-3, caspase-9 and poly(ADP-ribose) polymerase (PARP) cleavage occurred following AMPs treatment in C6 cells. These results suggested that the induction of apoptosis via the mitochondrial pathway was involved in the anti-proliferative activity of AMPs against glioma C6 cells. |
Databáze: | OpenAIRE |
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