Synthetic donor and acceptor splice sites function in an RNA polymerase B (II) transcription unit
| Autor: | G Rautmann, R Breathnach, M J Gait, H W Matthes |
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| Rok vydání: | 1984 |
| Předmět: |
Transcription
Genetic Ovalbumin RNA Splicing Oligonucleotides RNA polymerase II General Biochemistry Genetics and Molecular Biology Transcription (biology) Consensus sequence Animals splice Molecular Biology RNA polymerase II holoenzyme Splice site mutation Base Sequence General Immunology and Microbiology biology General Neuroscience Intron Molecular biology Globins Genes Oligodeoxyribonucleotides biology.protein RNA Polymerase II Rabbits Transcription factor II D Chickens Plasmids Research Article |
| Zdroj: | The EMBO Journal. 3:2021-2028 |
| ISSN: | 0261-4189 |
| Popis: | We have synthesised a 32-bp oligonucleotide containing sequences conforming to the consensus sequences for donor and acceptor splice sites. The oligonucleotide has been inserted into an RNA polymerase B (II) transcription unit and the resulting recombinant used to study the splicing mechanism. Our findings are as follows: (i) the synthetic sites function when separated by several different prokaryotic or eukaryotic DNA fragments providing bulk intron sequence, (ii) intron size need not be greater than 29 bp, (iii) an AG dinucleotide 11 bp upstream from the invariant AG of an acceptor splice site renders the latter non-functional, and (iv) sequence changes distant from splice sites can affect the efficiency of their utilisation. |
| Databáze: | OpenAIRE |
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