Estradiol modulated colorectal cancer stem cells bioactivity and interaction with endothelial cells
| Autor: | Jafar Rezaie, Mahdi Ahmadi, Yasaman Mirhosseini, Arezoo Rezaie Nezhad Zamani, Reza Rahbarghazi, Cigir Biray Avci, Emel Sokullu, Shirin Saberianpour, Amir Mehdizadeh, Hesam Saghaei Bagheri, Morteza Heidarzadeh, Ayda Pouyafar, Mehdi Talebi, Farzaneh Fathi |
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| Přispěvatelé: | Ege Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Human cancer stem cells Cell Survival Cell Apoptosis Survival and proliferation 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology Endothelial affinity 03 medical and health sciences Paracrine signalling 0302 clinical medicine Cell Movement Cancer stem cell medicine Humans MTT assay General Pharmacology Toxicology and Pharmaceutics Migration Paracrine activity Cell Proliferation Migration Assay Estradiol Chemistry Endothelial Cells Cancer Cell migration General Medicine medicine.disease Molecular biology 030104 developmental biology medicine.anatomical_structure Receptors Estrogen Neoplastic Stem Cells Stem cell Colorectal Neoplasms HT29 Cells |
| Popis: | This study aimed to evaluate the modulatory role of sex-related hormone estradiol on cancer stem cells with the origin of colorectal adenocarcinoma in vitro. Cancer stem cells were incubated with 100 nM estradiol for 48 h. the cell survival rate was analyzed using the MTT assay. Immunocytochemistry staining of Ki-67 and Inhibin and Apoptosis PCR array were done to measure proliferation/apoptosis. Cell migration was monitored via the Transwell Migration assay. the expression of exosome biogenesis genes was measured using a real-time PCR assay. the fatty acid profile was monitored using gas chromatography. the level of FAK, SQSTM1, ER, and SIRT1 was examined using Western blotting. Cancer stem-endothelial cell interaction was investigated using Surface Plasmon Resonance assay. Data showed no significant differences in cancer stem cell viability and proliferation between control and estradiol-treated groups (p 0.05). PCR array highlighted the up-regulation of both proand anti-apoptosis effectors in the treatment group compared to the control cells (p < 0.05). Cell migration capacity was increased after treatment with estradiol (p < 0.001). Both exocytosis and exosome biogenesis were decreased in cancer stem cells exposed to estradiol (p < 0.05). Data showed the reduction of palmitic acid, and increase of Palmitoleic and Linolenic acids in estradiol-treated cells. Estrogen induced estrogen receptor, SQSTM1 proteins and decreased SIRT1 factor after 48 h. Surface Plasmon Resonance revealed the suppression of cancer stemendothelial cell interaction and affinity. Estradiol could change the migration, juxtacrine and paracrine activities of cancer stem cells, showing the importance of sex-related hormones in the dynamic of cancer development. Tabriz University of Medical Sciences [IR.TBZMED.REC.1399.354] This work was supported by a grant (IR.TBZMED.REC.1399.354) from Tabriz University of Medical Sciences. Authors wish to thank personnel of Stem Cell Research Center for guidance and help. |
| Databáze: | OpenAIRE |
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