Radiation-induced protein fragmentation and inactivation in liquid and solid aqueous solutions. Role of OH and electrons
| Autor: | Marilyne Audette-Stuart, Michel Potier, Chantal Houée-Levin |
|---|---|
| Přispěvatelé: | Laboratoire de Chimie Physique D'Orsay (LCPO), Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2005 |
| Předmět: |
0303 health sciences
Radiation Aqueous solution 030302 biochemistry & molecular biology Radiation induced Electron Photochemistry 03 medical and health sciences chemistry.chemical_compound Polymerization chemistry Fragmentation (mass spectrometry) Molecule Hydroxyl radical [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] Irradiation ComputingMilieux_MISCELLANEOUS 030304 developmental biology |
| Zdroj: | Radiation Physics and Chemistry Radiation Physics and Chemistry, Elsevier, 2005, 72 (2-3), pp.301-306 |
| ISSN: | 0969-806X |
| DOI: | 10.1016/j.radphyschem.2003.12.060 |
| Popis: | Irradiation of proteins in diluted liquid aqueous solutions produces cleavages and polymerizations of the peptidic chains. In frozen solutions, fragmentation is observed but polymerization products are absent. Loss of activity occurs in both cases. In the solid state, yields of fragmentation do not vary with the quantity of water. The use of scavengers indicates that hydroxyl radical does not contribute significantly to fragmentation and to inactivation in the solid state. Electrons within the water molecules closely associated with the protein are involved in the processes leading to protein fragmentation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect