Mineral fiber-induced leukocyte activation: the role of intra- and extracellular calcium
| Autor: | Marjo Ruotsalainen, Jonne Naarala, Kai Savolainen |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Asbestos
Serpentine Fura-2 Cell Survival Neutrophils chemistry.chemical_element Calcium Lymphocyte Activation Toxicology Calcium in biology chemistry.chemical_compound medicine Extracellular Humans Egtazic Acid Calcimycin Respiratory Burst Dose-Response Relationship Drug Calcium channel Quartz General Medicine Respiratory burst N-Formylmethionine Leucyl-Phenylalanine EGTA Spectrometry Fluorescence Verapamil chemistry Biochemistry Luminescent Measurements Biophysics Tetradecanoylphorbol Acetate Reactive Oxygen Species medicine.drug |
| Zdroj: | Toxicology Letters. 78:195-205 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/0378-4274(95)03255-j |
| Popis: | The role of intra- and extracellular calcium in the activation of human polymorphonuclear leukocytes (PMNL) to produce reactive oxygen metabolites (ROM) were studied by using soluble, formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), or particulate stimuli, quartz or chrysotile. A calcium channel inhibitor, verapamil, attenuated only quartz-induced elevation of free intracellular calcium ([Ca2+]i) and ROM production. Likewise, ethyleneglycol-bis (aminoethyl ether) tetraacetic acid (EGTA) attenuated quartz-, chrysotile- and fMLP-induced elevation of [Ca2+]i and ROM production. It also inhibited PMA-induced ROM production. A calcium ionophore, A23187 amplified ROM production by all of these stimuli. These results suggest that both intra- and extra-cellular calcium are required for the full activation of respiratory burst by soluble and particulate stimuli in human PMNL. |
| Databáze: | OpenAIRE |
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