Author Correction: The Kunitz Domain I of Hepatocyte Growth Factor Activator Inhibitor-2 Inhibits Matriptase Activity and Invasive Ability of Human Prostate Cancer Cells
| Autor: | Hsin-Fang Tu, Chun-Jung Ko, Pei-Wen Hsiao, Hsiang-Po Huang, Chen-Hsin Teng, Tai-Shan Cheng, Hsin-Ying Lin, Ming-Shyue Lee, Hsin-Hsien Lin, Shao-Wei Lan, Chung-Hsin Chen, Shang-Ru Wu, Ya-Ting Tu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Serine Proteinase Inhibitors lcsh:Medicine Human prostate Text mining Protein Domains Cell Movement Cell Line Tumor Animals Humans Matriptase Amino Acid Sequence lcsh:Science Author Correction Mice Inbred BALB C Membrane Glycoproteins Multidisciplinary Sequence Homology Amino Acid biology business.industry lcsh:R Serine Endopeptidases Prostatic Neoplasms HEPATOCYTE GROWTH FACTOR ACTIVATOR INHIBITOR HEK293 Cells Proteolysis Cancer cell biology.protein Cancer research lcsh:Q RNA Interference Kunitz domain business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-2 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-60001-w |
| Popis: | Dysregulation of pericellular proteolysis is often required for tumor invasion and cancer progression. It has been shown that down-regulation of hepatocyte growth factor activator inhibitor-2 (HAI-2) results in activation of matriptase (a membrane-anchored serine protease), human prostate cancer cell motility and tumor growth. In this study, we further characterized if HAI-2 was a cognate inhibitor for matriptase and identified which Kunitz domain of HAI-2 was required for inhibiting matriptase and human prostate cancer cell motility. Our results show that HAI-2 overexpression suppressed matriptase-induced prostate cancer cell motility. We demonstrate that HAI-2 interacts with matriptase on cell surface and inhibits matriptase proteolytic activity. Moreover, cellular HAI-2 harnesses its Kunitz domain 1 (KD1) to inhibit matriptase activation and prostate cancer cell motility although recombinant KD1 and KD2 of HAI-2 both show an inhibitory activity and interaction with matriptase protease domain. The results together indicate that HAI-2 is a cognate inhibitor of matriptase, and KD1 of HAI-2 plays a major role in the inhibition of cellular matritptase activation as well as human prostate cancer invasion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|