Efficacy of protease inhibitor from marine Streptomyces sp. VITBVK2 against Leishmania donovani - An in vitro study
| Autor: | Veena Sreedharan, K.V. Bhaskara Rao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Geologic Sediments medicine.medical_treatment 030106 microbiology Immunology Leishmania donovani Antiprotozoal Agents Biology Streptomyces Microbiology 03 medical and health sciences Inhibitory Concentration 50 Casein medicine Chymotrypsin Protease Inhibitors Trypsin Amastigote Phylogeny Protease Macrophages Caseins General Medicine biology.organism_classification Flow Cytometry Protease inhibitor (biology) 030104 developmental biology Infectious Diseases Glucose biology.protein Microscopy Electron Scanning Leishmaniasis Visceral Parasitology medicine.drug Peptide Hydrolases |
| Zdroj: | Experimental parasitology. 174 |
| ISSN: | 1090-2449 |
| Popis: | In the present study the leishmanicidal effect of potential protease inhibitor producing marine actinobacterial isolate has been investigated against Leishmania donovani, the causative agent of visceral leishmaniasis. Among 89 marine actinobacteria isolated from a salt pan in Kanyakumari, only one isolate (BVK2) showed 97% of protease inhibition activity against trypsin. Moderate to high protease inhibitor activity was shown by isolate BVK2 on proteinase (30%) and chymotrypsin (85%). In optimization study for protease inhibitor production glucose as carbon source and casein as nitrogen source showed the best activity. In the in-vitro Fluorescence-activated cell sorting (FACS) assay, 100 μg/ml of BVK2 extract was active against amastigotes in infected J774A.1 macrophages and showed 87% of parasitic inhibition. The isolate BVK2 showed significant anti-parasitic activity with an IC50 of 27.1 μg/ml after double doses were administered. The potential isolate was identified by molecular 16S rRNA gene sequencing as Streptomyces sp. VITBVK2. The results obtained suggest that the marine actinobacterial extract which have novel metabolites can be considered as a potential source for the development of drugs. |
| Databáze: | OpenAIRE |
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