Modulation of organ ICAM-1 expression during IV-TPN with glutamine and bombesin
| Autor: | Cheryl D. Johnson, Kazuhiko Fukatsu, Ben L. Zarzaur, Andrew H. Lundberg, Keith M. Hanna, Kenneth A. Kudsk, Yong Wu, Henry G. Wilcox |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Parenteral Nutrition Ratón medicine.medical_treatment Glutamine Peptide hormone Biology Critical Care and Intensive Care Medicine Kidney chemistry.chemical_compound Mice Internal medicine Intensive care medicine Animals Intestinal Mucosa Muscle Skeletal Mice Inbred ICR Body Weight Bombesin Interleukin Intercellular Adhesion Molecule-1 Interleukin-10 Intestines medicine.anatomical_structure Endocrinology Cytokine chemistry Liver Emergency Medicine Interleukin-4 |
| Zdroj: | Shock (Augusta, Ga.). 15(1) |
| ISSN: | 1073-2322 |
| Popis: | The gut primes neutrophils (PMNs) during injury, which can then induce distant organ damage after a second insult. ICAM-1 is an important adhesion molecule in PMN attachment to the vascular endothelium. Parenteral nutrition (TPN) decreases gut levels of interleukin (IL)-4 and IL-10, two cytokines that are normal inhibitors of ICAM-1 expression. TPN also increases gut ICAM-1 expression and PMN accumulation. Since glutamine (GLN) and bombesin (BBS) prevent TPN-associated impairment of mucosal immunity, we hypothesized that GLN and BBS would modulate organ ICAM-1 expression in association with normalization of IL-4 and IL-10 levels. Forty-four mice were fed chow, TPN, or GLN-TPN (isonitrogenous 2% GLN-enriched TPN). After 5 days of diets, ICAM-1 expression was quantified in organs using the dual radiolabeled monoclonal antibody technique. In the next experiment, 29 mice were fed chow, TPN, or BBS-TPN (BBS 15 microg/kg TID) for 5 days to measure organ ICAM-1 expression. Total IL-4 and IL-10 levels were measured with ELISA from intestinal homogenates of another set of 52 mice fed chow, TPN, GLN-TPN, or BBS-TPN. TPN significantly increased ICAM-1 expression in the lung, kidney, and intestine compared with chow mice. GLN-TPN decreased intestinal, but not lung, ICAM-1 expression, while BBS-TPN reduced pulmonary, but not gut, ICAM-1 levels. GLN- and BBS-TPN returned gut IL-4 levels to normal, but failed to increase IL-10 levels. GLN and BBS had different effects on organ ICAM-1 expression induced by lack of enteral nutrition. Mechanisms other than recovery of IL-4 alone may be responsible for gut ICAM-1 expression. |
| Databáze: | OpenAIRE |
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