An improved vitrification protocol for equine immature oocytes, resulting in a first live foal
Autor: | Cyrillus Ververs, T. Vullers, M.C. Van De Velde, Nerea Ortiz-Escribano, Lynn Vandenberghe, A. Van Soom, Katrien Smits, E. Van den Abbeel, Jan Govaere, C. De Schauwer, Henri Woelders, O. Bogado Pascottini, K. Roels |
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Rok vydání: | 2018 |
Předmět: |
Glycerol
Veterinary medicine cumulus cells Horse OPS Cryopreservation Embryo Culture Techniques 0302 clinical medicine Human fertilization Pregnancy FERTILIZATION Vitrification Fertilisation 030219 obstetrics & reproductive medicine HORSE OOCYTES Embryo 04 agricultural and veterinary sciences General Medicine Embryo transfer horse medicine.anatomical_structure Female Tissue Preservation Animal Breeding & Genomics medicine.medical_specialty Cumulus cells ORGANIZATION Biology BOVINE OOCYTES ICSI MATURATION Andrology 03 medical and health sciences EMBRYOS medicine Animals oocytes Dimethyl Sulfoxide Blastocyst Horses Veterinary Sciences Fokkerij & Genomica PROGRESS Gynecology Dose-Response Relationship Drug 0402 animal and dairy science CRYOPRESERVATION Oocyte Embryo Transfer 040201 dairy & animal science vitrification In Vitro Oocyte Maturation Techniques WIAS Oocytes |
Zdroj: | Equine Veterinary Journal EQUINE VETERINARY JOURNAL Equine Veterinary Journal, 50(3), 391-397 Equine veterinary journal Equine Veterinary Journal 50 (2018) 3 |
ISSN: | 0425-1644 |
DOI: | 10.1111/evj.12747 |
Popis: | SummaryBackground The success rate for vitrification of immature equine oocytes is low. Although vitrified-warmed oocytes are able to mature, further embryonic development appears to be compromised. Objectives The aim of this study was to compare two vitrification protocols, and to examine the effect of the number of layers of cumulus cells surrounding the oocyte during vitrification of immature equine oocytes. Study design Experimental in vitro and in vivo trials. Methods Immature equine oocytes were vitrified after a short exposure to high concentrations of cryoprotective agents (CPAs), or a long exposure to lower concentrations of CPAs. In Experiment 1, the maturation of oocytes surrounded by multiple layers of cumulus cells (CC oocytes) and oocytes surrounded by only corona radiata (CR oocytes) was investigated. In Experiment 2, spindle configuration was determined for CR oocytes vitrified using the two vitrification protocols. In Experiment 3, further embryonic development was studied after fertilisation and culture. Embryo transfer was performed in a standard manner. Results Similar nuclear maturation rates were observed for CR oocytes vitrified using the long exposure and non-vitrified controls. Furthermore, a lower maturation rate was obtained for CC oocytes vitrified with the short exposure compared to control CR oocytes (p = 0.001). Both vitrification protocols resulted in significantly higher rates of aberrant spindle configuration than the control groups (p |
Databáze: | OpenAIRE |
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