An improved vitrification protocol for equine immature oocytes, resulting in a first live foal

Autor: Cyrillus Ververs, T. Vullers, M.C. Van De Velde, Nerea Ortiz-Escribano, Lynn Vandenberghe, A. Van Soom, Katrien Smits, E. Van den Abbeel, Jan Govaere, C. De Schauwer, Henri Woelders, O. Bogado Pascottini, K. Roels
Rok vydání: 2018
Předmět:
Glycerol
Veterinary medicine
cumulus cells
Horse
OPS
Cryopreservation
Embryo Culture Techniques
0302 clinical medicine
Human fertilization
Pregnancy
FERTILIZATION
Vitrification
Fertilisation
030219 obstetrics & reproductive medicine
HORSE OOCYTES
Embryo
04 agricultural and veterinary sciences
General Medicine
Embryo transfer
horse
medicine.anatomical_structure
Female
Tissue Preservation
Animal Breeding & Genomics
medicine.medical_specialty
Cumulus cells
ORGANIZATION
Biology
BOVINE OOCYTES
ICSI
MATURATION
Andrology
03 medical and health sciences
EMBRYOS
medicine
Animals
oocytes
Dimethyl Sulfoxide
Blastocyst
Horses
Veterinary Sciences
Fokkerij & Genomica
PROGRESS
Gynecology
Dose-Response Relationship
Drug
0402 animal and dairy science
CRYOPRESERVATION
Oocyte
Embryo Transfer
040201 dairy & animal science
vitrification
In Vitro Oocyte Maturation Techniques
WIAS
Oocytes
Zdroj: Equine Veterinary Journal
EQUINE VETERINARY JOURNAL
Equine Veterinary Journal, 50(3), 391-397
Equine veterinary journal
Equine Veterinary Journal 50 (2018) 3
ISSN: 0425-1644
DOI: 10.1111/evj.12747
Popis: SummaryBackground The success rate for vitrification of immature equine oocytes is low. Although vitrified-warmed oocytes are able to mature, further embryonic development appears to be compromised. Objectives The aim of this study was to compare two vitrification protocols, and to examine the effect of the number of layers of cumulus cells surrounding the oocyte during vitrification of immature equine oocytes. Study design Experimental in vitro and in vivo trials. Methods Immature equine oocytes were vitrified after a short exposure to high concentrations of cryoprotective agents (CPAs), or a long exposure to lower concentrations of CPAs. In Experiment 1, the maturation of oocytes surrounded by multiple layers of cumulus cells (CC oocytes) and oocytes surrounded by only corona radiata (CR oocytes) was investigated. In Experiment 2, spindle configuration was determined for CR oocytes vitrified using the two vitrification protocols. In Experiment 3, further embryonic development was studied after fertilisation and culture. Embryo transfer was performed in a standard manner. Results Similar nuclear maturation rates were observed for CR oocytes vitrified using the long exposure and non-vitrified controls. Furthermore, a lower maturation rate was obtained for CC oocytes vitrified with the short exposure compared to control CR oocytes (p = 0.001). Both vitrification protocols resulted in significantly higher rates of aberrant spindle configuration than the control groups (p
Databáze: OpenAIRE
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