Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
| Autor: | Lianpeng Wu, Jinhua Liu, Siying Teng, Yanyu Shi, Xinmin Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Detection limit Article Subject 010401 analytical chemistry Dna concentration Loop-mediated isothermal amplification food and beverages lcsh:TX341-641 Biology biology.organism_classification 01 natural sciences DNA extraction 0104 chemical sciences 03 medical and health sciences Human health 030104 developmental biology Animal science TaqMan Food science Safety Risk Reliability and Quality Routine analysis lcsh:Nutrition. Foods and food supply Food Science Nyctereutes procyonoides |
| Zdroj: | Journal of Food Quality, Vol 2017 (2017) |
| ISSN: | 0146-9428 |
| DOI: | 10.1155/2017/9319035 |
| Popis: | Raccoon dog (Nyctereutes procyonoides) is an economically important animal used for fur production, but consuming its meat is injurious to human health. Currently, no rapid and sensitive method for detecting raccoon dog meat in meat mixtures is available. In this study, we developed an easily applicable, rapid, and economically feasible method for identifying the presence of raccoon dog in meat mixtures based on loop-mediated isothermal amplification (LAMP). Four sets of LAMP primers were tested at different temperatures, and the primers that worked best at 62°C (set 2) were determined. In the LAMP assay, there was no cross-reactivity with the meat procured from other species of animals and the detection limit of DNA concentration was 0.1 pg·μL−1, slightly higher than TaqMan real-time PCR (0.01 pg·μL−1), but sensitivity of 0.1 pg·μL−1 complies with most requirements of routine analysis. Moreover, by the LAMP method, the meat mixtures containing more than 0.5% of the raccoon dog component were directly detected (without DNA extraction) in the supernatant isolated from the meat mixtures after performing repeated cycles of thawing and freezing of minced meat mixtures. Our results show that LAMP assay is a valuable, straightforward, and sensitive detection tool for identification of raccoon dog meat in mixtures. |
| Databáze: | OpenAIRE |
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