Heparin binding proteins from Leishmania (Viannia) braziliensis promastigotes
| Autor: | Ricardo L Azevedo-Pereira, Mirian Claudia de Souza Pereira, André L.S. Santos, Reginaldo Peçanha Brazil, Leny Toma, Maria de Fátima Madeira, Luzia Monteiro de Castro Côrtes, F.O.R. Oliveria-Junior, Carlos Roberto Alves |
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| Rok vydání: | 2007 |
| Předmět: |
Gel electrophoresis
General Veterinary biology Hemagglutination Protozoan Proteins Biological activity General Medicine biology.organism_classification Leishmania DNA-binding protein Molecular biology Leishmania braziliensis Affinity chromatography parasitic diseases Animals Parasite hosting Parasitology Lutzomyia Cell Adhesion Molecules |
| Zdroj: | Veterinary Parasitology. 145:234-239 |
| ISSN: | 0304-4017 |
| Popis: | We have examined the heparin binding proteins from Leishmania (Viannia) braziliensis promastigotes (HBP-Lb) by chromatography assays. The proposed strategy to isolate an enriched fraction of the HBP-Lb consisted of an association of the Triton X-114 method with affinity chromatography in heparin-Sepharose 4B column. SDS-PAGE analysis of the eluted proteins showed two main protein bands (65.0 and 54.5 kDa), while a single protein band was observed in native electrophoresis gel. The hemagglutination property of HBP-Lb over rabbit erythrocytes was confirmed up to 6.3 ± 0.5 μg of protein mL−1. Additionally, we have assayed the potential of HBP-Lb labeled with sulfo-NHS-LC-biotin in binding to nitrocellulose-immobilized gut proteins extracted of Lutzomyia intermedia and Lutzomyia whitmani. The results indicated a similar profile of five ligands (67.0, 62.1, 59.5, 56.0 and 47.5 kDa) in both studied Lutzomyia species. This is the first direct description of this class of protein in L. (V.) braziliensis with a suggestion of its biological activity in the interaction of Leishmania with Lutzomyia gut cells, which maybe a crucial step during this parasite's life cycle. |
| Databáze: | OpenAIRE |
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