Diamond Squid (Thysanoteuthis rhombus)-Derived Chondroitin Sulfate Stimulates Bone Healing within a Rat Calvarial Defect
Autor: | Jun-ichi Tamura, Yoshinao Z. Hosaka, Yuji Iwai, Masato Uehara |
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Jazyk: | angličtina |
Rok vydání: | 2013 |
Předmět: |
Male
Bone Regeneration Pharmaceutical Science regenerative medicine Calvaria Bone Morphogenetic Protein 4 Bone healing Bone morphogenetic protein Article Hydrogel Polyethylene Glycol Dimethacrylate chemistry.chemical_compound Osteogenesis Drug Discovery medicine BMP-4 Animals Chondroitin sulfate Rats Wistar Von Kossa stain Bone regeneration Pharmacology Toxicology and Pharmaceutics (miscellaneous) lcsh:QH301-705.5 chondroitin sulfate diamond squid (Thysanoteuthis rhombus) Chondroitin Sulfates Skull Mesenchymal stem cell Decapodiformes Mesenchymal Stem Cells Anatomy Molecular biology Rats Apposition medicine.anatomical_structure chemistry lcsh:Biology (General) Gelatin bone healing |
Zdroj: | Marine Drugs, Vol 11, Iss 12, Pp 5024-5035 (2013) Marine Drugs Volume 11 Issue 12 Pages 5024-5035 |
ISSN: | 1660-3397 |
Popis: | Chondroitin sulfate (CS) has been suggested to be involved in bone formation and mineralization processes. A previous study showed that squid-derived CS (sqCS) has osteoblastogenesis ability in cooperation with bone morphogenetic protein (BMP)-4 in vitro. However, in vivo, osteogenic potential has not been verified. In this study, we created a critical-sized bone defect in the rat calvaria and implanted sqCS-loaded gelatin hydrogel sponges (Gel) into the defect with or without BMP-4 (CS/BMP/Gel and CS/Gel, respectively). At 15 weeks, bone repair rate of CS/Gel-treated defects and CS/BMP/Gel-treated defects were 47.2% and 51.1%, respectively, whereas empty defects and defects with untreated sponges showed significantly less bone ingrowth. The intensity of von Kossa staining of the regenerated bone was less than that of the original one. Mineral apposition rates at 9 to 10 weeks were not significantly different between all treatment groups. Although bone repair was not completed, sqCS stimulated bone regeneration without BMP-4 and without external mesenchymal cells or preosteoblasts. Therefore, sqCS is a promising substance for promotion of osteogenesis. |
Databáze: | OpenAIRE |
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