Effects of Gamma Irradiation of Human Serum Samples from rVSVΔG-ZEBOV-GP (V920) Ebola Virus Vaccine Recipients on Plaque-Reduction Neutralization Assays
| Autor: | Joseph M. Antonello, Rick Nichols, Rebecca J. Grant-Klein, Sheri Dubey, Jakub K. Simon |
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| Rok vydání: | 2021 |
| Předmět: |
Phases of clinical research
Antibodies Viral medicine.disease_cause Neutralization Immunogenicity Vaccine Viral Envelope Proteins Neutralization Tests Virology parasitic diseases Humans Medicine Prospective Studies Ebola Vaccines Neutralizing antibody Ebola virus biology business.industry Immune Sera Vaccination Articles Vesiculovirus Hemorrhagic Fever Ebola Ebolavirus Serum samples Antibodies Neutralizing Healthy Volunteers Target dose Titer Infectious Diseases biology.protein population characteristics Parasitology business human activities Gamma irradiation |
| Zdroj: | Am J Trop Med Hyg |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.20-1055 |
| Popis: | Gamma irradiation (GI) is included in the CDC guidance on inactivation procedures to render a group of select agents and toxins nonviable. The Ebola virus falls within this group because it potentially poses a severe threat to public health and safety. To evaluate the impact of GI at a target dose of 50 kGy on neutralizing antibody titers induced by the rVSVΔG-ZEBOV-GP vaccine (V920), we constructed a panel of 48 paired human serum samples (GI-treated versus non–GI-treated) from healthy participants selected from a phase 3 study of V920 (study V920-012; NCT02503202). Neutralizing antibody titers were determined using a validated plaque-reduction neutralization test. GI of sera from V920 recipients was associated with approximately 20% reduction in postvaccination neutralizing antibody titers. GI was not associated with any change in pre-vaccination neutralizing antibody titers. |
| Databáze: | OpenAIRE |
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