Nonspecific cleavage of phi X174 RFI deoxyribonucleic acid by bleomycin
| Autor: | Robb E. Moses, Jack D. Love, Charles D. Liarakos |
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| Rok vydání: | 1981 |
| Předmět: |
Binding Sites
biology Chemical Phenomena HpaII viruses DNA Restriction Enzymes respiratory system Bleomycin Cleavage (embryo) Biochemistry chemistry.chemical_compound Restriction enzyme Chemistry PstI chemistry Covalent bond Duplex (building) DNA Viral biology.protein Electrophoresis Polyacrylamide Gel DNA Bacteriophage phi X 174 |
| Zdroj: | Biochemistry. 20(18) |
| ISSN: | 0006-2960 |
| Popis: | The covalently closed circular duplex deoxyribonucleic acid (DNA) of phi X174 underwent progressive conversion to nicked and linear DNA with increasing bleomycin/phi X174 RFI DNA molecule ratios. The formation of linear DNA (a double-strand break) occurred under limited reaction conditions as low as an average of 0.2 single-strand break/phi X174 RFI DNA molecule. As bleomycin-produced linear DNA was further fragmented by bleomycin, a broad distribution of DNA fragments without notable concentrations of unique size was formed. Restriction enzymes PstI and SstII did not generate discrete fragments from bleomycin-produced full-length linear phi X174 DNA, nor did bleomycin cleavage generate discrete fragments from HpaII or PstI digests of phi X174 RFI. These findings suggest that bleomycin does not act at a few specific sites on phi X174 RFI DNA. The single-strand nick appeared to be the preferred site for bleomycin action for a second cleavage in a phi X174 molecule. |
| Databáze: | OpenAIRE |
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