PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle
| Autor: | A Soltyk, J D Beggs, J D Friesen, S P Bjørn |
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| Rok vydání: | 1989 |
| Předmět: |
Transcription
Genetic RNA Splicing Saccharomyces cerevisiae Genes Fungal Molecular Sequence Data Biology Gene product Fungal Proteins Amino Acid Sequence DNA Fungal Molecular Biology Gene Antibodies Fungal Base Sequence Small Nuclear Ribonucleoprotein Particle C4A Cell Biology biology.organism_classification Ribonucleoproteins Small Nuclear Molecular biology Ribonucleoproteins RNA splicing AKT1S1 Precursor mRNA Research Article |
| Zdroj: | Molecular and cellular biology. 9(9) |
| ISSN: | 0270-7306 |
| Popis: | The PRP4 (RNA4) gene product is involved in nuclear mRNA processing in yeast cells; we have previously cloned the gene by complementation of a temperature-sensitive mutation. Sequence and transcript analyses of the cloned gene predicted the gene product to be a 52-kilodalton protein, which was confirmed with antibodies raised against the PRP4 gene product. These antibodies inhibited precursor mRNA splicing in vitro, demonstrating a direct role of PRP4 in splicing. Immunoprecipitations with the antibodies indicated that the PRP4 protein is associated with the U4/U6 small nuclear ribonucleoprotein particle. |
| Databáze: | OpenAIRE |
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