Rapid cryopreservation of small quantities of human spermatozoa by a self-prepared cryoprotectant without animal component
| Autor: | Shasha Liu, Yang Xian, Qingyuan Cheng, Huanxun Yue, Min Jiang, Xiao Liu, Bo Liu, Wenrui Zhao, Fuping Li |
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| Rok vydání: | 2021 |
| Předmět: |
Azoospermia
Cryopreservation Male Cryoprotectant Cryptozoospermia urogenital system Urology Motility General Medicine Biology medicine.disease Sperm Spermatozoa Andrology Endocrinology Cryoprotective Agents Freezing medicine Sperm Motility DNA fragmentation Animals Humans Fertility preservation Semen Preservation |
| Zdroj: | Andrologia. 54(2) |
| ISSN: | 1439-0272 |
| Popis: | Cryopreservation of small quantities of human spermatozoa whilst maintaining adequate post-thawing motility has been found an essential challenge for male fertility preservation. Therefore, the study used an effective, and convenient rapid-freezing method to freeze small amounts of human spermatozoa by adding self-prepared cryoprotectant (SPC) without animal component. In the feasibility experiment, no significant differences in progressive motility, normal sperm morphology, vitality or DNA fragmentation index between the conventional slow freezing and rapid freezing were realised. The present study prospectively analysed the effects of sperm freezing and resuscitation in 175 patients with severe oligozoospermia (sperm concentration |
| Databáze: | OpenAIRE |
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