Optimizing the performance of multiline-scanning confocal microscopy
| Autor: | Chun-Hung Weng, Kyu Young Han, Jialei Tang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science genetic structures Acoustics and Ultrasonics Optical sectioning business.industry Ranging Condensed Matter Physics Single excitation Photobleaching Article eye diseases Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention Optics Duty cycle Confocal microscopy law sense organs business |
| Zdroj: | J Phys D Appl Phys |
| ISSN: | 1361-6463 0022-3727 |
| DOI: | 10.1088/1361-6463/abc84b |
| Popis: | Line-scanning (LS) confocal microscopy provides high imaging speed and moderate optical sectioning strength, which makes it a useful tool for imaging various biospecimens ranging from living cells to fixed tissues. Conventional LS systems have only used a single excitation line and slit, and thus have not fully exploited the benefits of parallelization. Here we investigate the optical performance of multi-LS confocal microscopy (mLS) by employing a digital micro-mirror device that provides programmable patterns of the illumination beam and the detection slit. Through experimental results and optical simulations, we assess the depth discrimination of mLS under different optical parameters and compare it with multi-point systems such as spinning disk confocal microscopy (SDCM). Under the same illumination duty cycle, we find that mLS has better optical sectioning than SDCM at a high degree of parallelization. The optimized mLS provides a low photobleaching rate and video-rate imaging while its optical sectioning is similar to single LS confocal microscopy. |
| Databáze: | OpenAIRE |
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