A rapid gas chromatographic method for the determination of plasma polyamines and its application to the prediction of tumour response to chemotherapy
| Autor: | J.F.C. Wicks, Marie T. Bakowski, J.R. Trounce, P.A. Toseland |
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| Rok vydání: | 1981 |
| Předmět: |
Adult
Male Chromatography Gas Adolescent medicine.medical_treatment Clinical Biochemistry Spermine Tumour response Biochemistry Malignant disease chemistry.chemical_compound Neoplasms Polyamines medicine Humans Chemotherapy Chromatography Biochemistry (medical) Cancer General Medicine Middle Aged Prognosis medicine.disease Spermidine chemistry Putrescine Female Gas chromatography |
| Zdroj: | Clinica Chimica Acta. 110:273-286 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(81)90356-9 |
| Popis: | Isobutyloxycarbonyl derivatives of the polyamines, spermidine, spermine and putrescine in 1 ml plasma were analysed on a mixed phase, 1.5% SE-30, 0.15% FFAP, column by temperature-programmed gas chromatography using a nitrogen-sensitive glass bead detector. Levels in 60 normal subjects (mean +/- 2- S.D.) were 0.13 +/- 0.08 mumol/l for spermidine, 0.02 +/- 0.04 for spermine and less than or equal to 0.18 for putrescine. Elevated spermidine levels were found pre-treatment in only 20% of patients with advanced metastatic cancer, reflecting the lack of sensitivity of polyamines as markers for malignant disease. However, serial studies in 26 patients undergoing remission induction chemotherapy showed that a significant rise in plasma spermidine within 48 h correlated with subsequent clinical response (p less than 0.001). Tumour response to chemotherapy can be predicted by the assay of plasma polyamines. |
| Databáze: | OpenAIRE |
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