HCV-RNA qualitative assay based on transcription mediated amplification improves the detection of hepatitis C virus infection in patients on hemodialysis: Results from five hemodialysis units in central Greece
| Autor: | George Kyriakopoulos, Eftichios Patsidis, Ioannis Stefanidis, Paraskevi Mina, Kalliopi Zachou, Eleftheria Zervou, Lambros Sdrakas, Eirini I. Rigopoulou, Christos Rizos, Christos Syrganis, Nikolaos Tsianas, Georgios N. Dalekos, Christos Liaskos |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Transcription Genetic Hepacivirus Hepatitis C virus medicine.medical_treatment Transcription-mediated amplification urologic and male genital diseases medicine.disease_cause Sensitivity and Specificity End stage renal disease Flaviviridae Renal Dialysis Risk Factors Virology Prevalence Humans Medicine Greece biology business.industry Gene Amplification Reproducibility of Results virus diseases Middle Aged biology.organism_classification Hepatitis C female genital diseases and pregnancy complications digestive system diseases Infectious Diseases biology.protein Kidney Failure Chronic RNA Viral Female Hemodialysis Viral disease Antibody business |
| Zdroj: | Journal of Clinical Virology. 34:81-85 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2005.05.007 |
| Popis: | Background: End-stage renal disease patients (ESRD) on maintenance hemodialysis (HD) are at increased risk of acquiring hepatitis C virus (HCV) infection. An early and accurate diagnosis of HCV infection is important for the prevention of viral transmission and the management of ESRD patients on HD but conventional ELISA and PCR have often failed to reveal active HCV infection. Objectives: This study evaluated the prevalence of HCV infection in ESRD patients from all HD units in central Greece using a sensitive HCV-RNA transcription mediated amplification (TMA) assay and compared its sensitivity with that of anti-HCV ELISA. Study design: Anti-HCV antibody (third generation ELISA), HCV-RNA (TMA) and HCV genotypes (HCV TMA-LiPA) were determined in 366 ESRD Greek patients. Results: In total, 132 (36%) ESRD patients were HCV positive by ELISA or TMA; 44 by TMA alone, 16 by ELISA alone and 72 positive by both assays. More than half of the viraemic patients had genotype 3a. Conclusions: HCV-RNA (TMA) assay appears to increase the accuracy in the diagnosis of HCV infection in HD patients compared to the anti-HCV ELISA and could serve as an additional screening tool in these patients. |
| Databáze: | OpenAIRE |
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