A recombinant canine distemper virus expressing interleukin-7 enhances humoral immunity
| Autor: | Huanchun Chen, Min Cui, Ming Zhou, Xiao-geng Yan, Yixi Chen, Chen Chen, Zhen-fang Fu, Ling Zhao |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
030106 microbiology Antibodies Viral Vaccines Attenuated Virus 03 medical and health sciences Mice Immune system Dogs Immunity Virology Chlorocebus aethiops medicine Animals Distemper Distemper Virus Canine Vero Cells B-Lymphocytes Mice Inbred BALB C Mice Inbred ICR biology Canine distemper Immunogenicity Interleukin-7 Vaccination Germinal center Viral Vaccines T-Lymphocytes Helper-Inducer medicine.disease Germinal Center Antibodies Neutralizing Immunity Humoral 030104 developmental biology Humoral immunity biology.protein Female Antibody |
| Zdroj: | The Journal of general virology. 100(4) |
| ISSN: | 1465-2099 |
| Popis: | Canine distemper (CD) causes gastrointestinal and respiratory and/or neurological signs and results in high morbidity and mortality, remaining a threat to carnivores around the world. Live-attenuated vaccines have been widely used to reduce the number of CD outbreaks, but efforts are still needed to improve immune efficiency. Interleukin-7 (IL-7) has been reported to boost host immunity by recruiting follicle helper T (TFH) or germinal center (GC) B cells. Here, we constructed a recombinant canine distemper virus (rCDV) by reverse genetics and evaluated the properties of six intergenic sites for insertion of a foreign gene. We found that the P/M intergenic region was the optimal site to insert a foreign gene into the CDV genome. The effect of overexpressing IL-7 on rCDV immunogenicity was then evaluated in a mouse model. We found that mice immunized with rCDV-IL7 could not significantly enhance the maturation of dendritic cells (DCs) but significantly facilitated the generation of TFH cells, GC B cells and plasma cells (PCs), as well as the formation of GCs, consequently enhancing the production of CDV-specific neutralizing antibodies and total IgG. Together, these results suggested that the overexpression of IL-7 by rCDV could enhance humoral responses by activating the TFH-GC B-PC axis, which will help to improve vaccines for CD. |
| Databáze: | OpenAIRE |
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