Dysfunctional inhibitory muscarinic receptors mediate enhanced histamine release in isolated human bronchi
| Autor: | Roland Buhl, Cornelius Kortsik, Ignaz Wessler, Björn Hölper, Heinz Kilbinger, Charles James Kirkpatrick |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Lung Neoplasms Time Factors chemistry.chemical_element Bronchi In Vitro Techniques Muscarinic Agonists Calcium Pharmacology Inhibitory postsynaptic potential Histamine Release General Biochemistry Genetics and Molecular Biology Pulmonary Disease Chronic Obstructive chemistry.chemical_compound Forced Expiratory Volume Muscarinic acetylcholine receptor medicine Oxotremorine Humans General Pharmacology Toxicology and Pharmaceutics Lung cancer Calcimycin Aged COPD Ionophores Smoking General Medicine Middle Aged respiratory system medicine.disease Receptors Muscarinic Acetylcholine respiratory tract diseases chemistry Immunology Female Histamine medicine.drug |
| Zdroj: | Life Sciences. 80:2294-2297 |
| ISSN: | 0024-3205 |
| Popis: | In human airways mucosal mast cells are under the control of inhibitory muscarinic receptors. The described experiments tested, whether the inhibitory potency of two muscarinic receptor agonists (oxotremorine, acetylcholine) becomes impaired in advanced chronic obstructive pulmonary disease (COPD). Isolated human bronchi obtained from 26 patients with lung cancer were separated into two groups. Group 1 patients suffered from moderate COPD (mean FEV1 56%; range 34-71%; mean pack years of cigarette smoking 50, range 20-96; one non-smoker). Group 2 patients had no or only a mild form of COPD; mean FEV1 was 82% (62-97%) and the number of pack years 22 (6-45; 3 non-smoker). The calcium ionophore A23187 induced a maximal histamine release of 4100+/-870 pmol/g/5 min in group 1 bronchi, in contrast to only 1730+/-240 pmol/g/5 min in group 2 bronchi (p0.02). Oxotremorine (1 nmol/L) reduced the stimulated histamine release by 81+/-5% in group 2 bronchi, but did not produce a significant effect in group 1 bronchi (11+/-14%). In conclusion, the present experiments show an enhanced histamine release in advanced COPD, which can be explained by a dysfunction of inhibitory muscarinic receptors. |
| Databáze: | OpenAIRE |
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