Expansion of myeloid derived suppressor cells contributes to platelet activation by l-arginine deprivation during sars-cov-2 infection
| Autor: | Luisa Marchioni, Davide Mariotti, Alessandra Sacchi, Chiara Agrati, Gabriele Garotto, Eleonora Tartaglia, Simona Gili, Stefania Notari, Alessia Beccacece, Michele Bibas, Germana Grassi, Veronica Bordoni, Emanuele Nicastri, Giuseppe Ippolito, Rita Casetti, Eleonora Cimini |
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| Přispěvatelé: | Sacchi, A., Grassi, G., Notari, S., Gili, S., Bordoni, V., Tartaglia, E., Casetti, R., Cimini, E., Mariotti, D., Garotto, G., Beccacece, A., Marchioni, L., Bibas, M., Nicastri, E., Ippolito, G., Agrati, C. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Arginine QH301-705.5 MDSC L-Arginine Pathogenesis Young Adult Immunopathology Humans Myeloid-Derived Suppressor Cell Platelet Platelet activation Biology (General) Aged platelet Aged 80 and over Chemistry Myeloid-Derived Suppressor Cells Brief Report COVID-19 Thrombosis General Medicine Middle Aged Platelet Activation In vitro Immunology Thrombosi Myeloid-derived Suppressor Cell Female Ex vivo Human |
| Zdroj: | Cells, Vol 10, Iss 2111, p 2111 (2021) Cells |
| Popis: | Massive platelet activation and thrombotic events characterize severe COVID-19, highlighting their critical role in SARS-CoV-2-induced immunopathology. Since there is a well-described expansion of myeloid-derived suppressor cells (MDSC) in severe COVID-19, we evaluated their possible role in platelet activation during SARS-CoV-2 infection. During COVID-19, a lower plasmatic L-arginine level was observed compared to healthy donors, which correlated with MDSC frequency. Additionally, activated GPIIb/IIIa complex (PAC-1) expression was higher on platelets from severe COVID-19 patients compared to healthy controls and inversely correlated with L-arginine plasmatic concentration. Notably, MDSC were able to induce PAC-1 expression in vitro by reducing L-arginine concentration, indicating a direct role of PMN-MDSC in platelet activation. Accordingly, we found a positive correlation between ex vivo platelet PAC-1 expression and PMN-MDSC frequency. Overall, our data demonstrate the involvement of PMN-MDSC in triggering platelet activation during COVID-19, highlighting a novel role of MDSC in driving COVID-19 pathogenesis. |
| Databáze: | OpenAIRE |
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