Characterization of a hepatitis A virus strain suitable for vaccine production
| Autor: | Arie J. Zuckerman, Anna Prugnola, Hemda Garelick, Vittoria Pellegrini, Nicoletta Fineschi, Filippo Cavalieri |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Viral Hepatitis Vaccines
viruses Immunoblotting Molecular Sequence Data Restriction Mapping Genome Viral Hepatitis A Antigens Biology medicine.disease_cause Genome Polymerase Chain Reaction law.invention Cell Line Capsid law Sequence Homology Nucleic Acid medicine Humans Amino Acid Sequence Hepatovirus Cloning Molecular Gene Escherichia coli Antigens Viral Antiserum Hepatitis A Vaccines Vaccines Synthetic Hepatology Strain (chemistry) Base Sequence Viral Vaccines biochemical phenomena metabolism and nutrition Virology Inactivated vaccine Recombinant DNA Capsid Proteins |
| Zdroj: | Journal of hepatology. 13 |
| ISSN: | 0168-8278 |
| Popis: | A novel isolate of hepatitis A virus, obtained from a clinical sample, has been adapted to grow on cultured human diploid cells. Growth and purification parameters have been optimized to obtain conditions suitable for the development of an inactivated vaccine. The entire viral genome was molecularly cloned, and the gene encoding the VP3 capsid protein was expressed in Escherichia coli. The resulting recombinant VP3 was used to obtain rabbit antisera which recognize the denatured protein in purified virion preparations. Nucleotide sequencing data are presented and compared to known sequences of different strains. |
| Databáze: | OpenAIRE |
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