Sulforaphane inhibits the expression of interleukin-6 and interleukin-8 induced in bronchial epithelial IB3-1 cells by exposure to the SARS-CoV-2 Spike protein

Autor: Jessica Gasparello, Brunella Grigolo, Elisabetta D'Aversa, Laura Gambari, Chiara Papi, Roberto Gambari, Monica Borgatti, Alessia Finotti
Rok vydání: 2021
Předmět:
Chemokine
sulforaphane
Pharmaceutical Science
Apoptosis
Spike
chemistry.chemical_compound
0302 clinical medicine
Isothiocyanates
Drug Discovery
Medicine
IL-6
interleukin-6
COVID-19
coronavirus disease 2019
SFN
sulforaphane
nutraceuticals
IL-8
interleukin-8
Regulation of gene expression
0303 health sciences
biology
Anti-Inflammatory Agents
Non-Steroidal
ARDS
Acute Respiratory Distress Syndrome
Up-Regulation
Cytokine release syndrome
Sulfoxides
030220 oncology & carcinogenesis
Spike Glycoprotein
Coronavirus
Molecular Medicine
Chemokines
medicine.symptom
Cytokine Release Syndrome
Bronchi
Inflammation
Article
NO
Cell Line
03 medical and health sciences
Humans
Interleukin 8
Interleukin 6
030304 developmental biology
Pharmacology
RT-qPCR
reverse transcription quantitative polymerase-chain reaction
Interleukin-6
SARS-CoV-2
business.industry
Interleukin-8
COVID-19
biomarkers
medicine.disease
SARS-CoV-2
Severe Acute Respiratory Syndrome Coronavirus 2
Gene Expression Regulation
Complementary and alternative medicine
chemistry
inflammation
Immunology
biology.protein
S-protein
Spike-protein
business
Cytokine storm
Sulforaphane
Zdroj: Phytomedicine
ISSN: 0944-7113
DOI: 10.1016/j.phymed.2021.153583
Popis: Background A key clinical feature of COVID-19 is a deep inflammatory state known as “cytokine storm” and characterized by high expression of several cytokines, chemokines and growth factors, including IL-6 and IL-8. A direct consequence of this inflammatory state in the lungs is the Acute Respiratory Distress Syndrome (ARDS), frequently observed in severe COVID-19 patients. Cytokine storm is associated with severe forms of COVID-19 and poor prognosis for COVID-19 patients. Sulforaphane (SFN), one of the main components of Brassica oleraceae L. (Brassicaceae or Cruciferae), is known to possess anti-inflammatory effects in tissues from several organs, among which joints, kidneys and lungs. Purpose The objective of the present study was to determine whether SFN is able to inhibit IL-6 and IL-8, two key molecules involved in the COVID-19 cytokine storm. Methods The effects of SFN were studied in vitro on bronchial epithelial IB3-1 cells exposed to the SARS-CoV-2 Spike protein (S-protein). The anti-inflammatory activity of SFN on IL-6 and IL-8 expression has been evaluated by RT-qPCR and Bio-Plex analysis. Results In our study SFN inhibits, in cultured IB3-1 bronchial cells, the gene expression of IL-6 and IL-8 induced by SARS-CoV-2. This represents the proof-of-principle that SFN may modulate the release of some key proteins of the COVID-19 cytokine storm. Conclusion The control of the cytokine storm is one of the major issues in the management of COVID-19 patients. Our study demonstrates that SFN can be employed in protocols useful to control hyperinflammatory state associated with SARS-CoV-2 infection.
Graphical abstract Image, graphical abstract
Databáze: OpenAIRE
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