Antioxidant activity from extra virgin olive oil via inhibition of hydrogen peroxide-mediated NADPH-oxidase 2 activation
| Autor: | Francesca Pagano, Daniele Pastori, Cristina Nocella, Roberto Monticolo, Lucia Stefanini, Roberto Cangemi, Francesco Violi, Simona Bartimoccia, Roberto Carnevale, Vittoria Cammisotto, Alessandra D'Amico |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Blood Platelets
Platelets Antioxidant Endocrinology Diabetes and Metabolism medicine.medical_treatment 030204 cardiovascular system & hematology Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine NOX2 medicine Humans Vitamin E Platelet 030212 general & internal medicine Gallic acid Food science Hydrogen peroxide Olive Oil Scavenger activity Nutrition and Dietetics NADPH oxidase biology EVOO Polyphenols Hydrogen Peroxide Catalase Healthy Volunteers chemistry Polyphenol Dietary Supplements NADPH Oxidase 2 biology.protein |
| Popis: | Objectives Extra virgin olive oil (EVOO) supplementation is associated with a significant reduction in cardiovascular disease but the underlying mechanism is still unclear. Methods In platelets that were taken from healthy subjects (n = 5), agonist-induced hydrogen peroxide (H 2 O 2 ) production and NADPH oxidase 2 (NOX2) activation in the presence of or without catalase, which catabolizes H 2 O 2 , were investigated. Platelet H 2 O 2 production, NOX2 activation, EVOO vitamin E, and total polyphenols as well as EVOO's ability to scavenge H 2 O 2 were also measured. Results Platelet NOX2 activation and H 2 O 2 production were significantly inhibited in catalase-treated platelets and platelets that were incubated with five different EVOOs. The EVOO content of vitamin E was 53 to 223 mg/kg and total polyphenols 145 to 392 mg/L Gallic acid equivalent. EVOOs quenched in vitro H 2 O 2 by 39 to 62%, which is an effect that is significantly correlated with vitamin E and total polyphenol concentrations ( R = 0.688; P R = 0.541; P Conclusions This in vitro study provides the first evidence that EVOO downregulates platelet H 2 O 2 and in turn NOX2 activity via H 2 O 2 scavenging. |
| Databáze: | OpenAIRE |
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