Increasing surface enhanced Raman spectroscopy effect of RNA and DNA components by changing the pH of silver colloidal suspensions
| Autor: | Oliva M. Primera-Pedrozo, Samuel P. Hernández-Rivera, Hilsamar Félix-Rivera, Jorge Castellanos, Gabriela Rodriguez, Oscar Resto |
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| Rok vydání: | 2012 |
| Předmět: |
Silver
Surface Properties Reducing agent Inorganic chemistry Metal Nanoparticles Nanoparticle DNA Surface-enhanced Raman spectroscopy Spectrum Analysis Raman Atomic and Molecular Physics and Optics Silver nanoparticle Analytical Chemistry chemistry.chemical_compound Sodium borohydride symbols.namesake chemistry Chemical engineering Sodium citrate symbols RNA Colloids Surface charge Raman spectroscopy Instrumentation Spectroscopy |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 87:77-85 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2011.11.012 |
| Popis: | This work focused on establishing the parameters for enhancing the Raman signals of DNA and RNA constituents: nitrogenous bases, nucleosides and nucleotides, using metallic nanoparticles as surface enhanced Raman scattering substrates. Silver nanospheres were synthesized using sodium borohydride as a reducing agent and sodium citrate as a capping agent. The prepared nanoparticles had a surface plasmon band at ∼384nm and an average size of 12±3nm. The nanoparticles' surface charge was manipulated by changing the pH of the Ag colloidal suspensions in the range of 1-13. Low concentrations as 0.7μM were detected under the experimental conditions. The optimum pH values were: 7 for adenine, 9 for AMP, 5 for adenosine, 7 for dAMP and 11 for deoxyadenosine. |
| Databáze: | OpenAIRE |
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