Tomato floral induction and flower development are orchestrated by the interplay between gibberellin and two unrelated microRNA-controlled modules
| Autor: | Lázaro Eustáquio Pereira Peres, Pollyanna Castilho, Eder Marques da Silva, Geraldo Felipe Ferreira e Silva, Fabio Tebaldi Silveira Nogueira, Isabel López-Díaz, Airton C. Junior, Marcela Morato Notini, Mateus H. Vicente, Nan Jiang, Erich Grotewold, Frederico Almeida de Jesus, Esther Carrera, João Paulo Oliveira Corrêa |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine LANCEOLATE Flowering time Physiology Meristem Plant Science Flowers Biology 01 natural sciences Models Biological 03 medical and health sciences Solanum lycopersicum microRNA Inflorescence Transcription factor Plant Proteins TOMATE Gibberellin (GA) MiR319 fungi food and beverages biology.organism_classification Gibberellins Cell biology MiR156 Shoot apex AP-1 transcription factor MicroRNAs 030104 developmental biology Mutation Gibberellin Solanum 010606 plant biology & botany |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| DOI: | 10.1111/nph.15492 |
| Popis: | [EN] Age-regulated microRNA156 (miR156) and targets similarly control the competence to flower in diverse species. By contrast, the diterpene hormone gibberellin (GA) and the microRNA319-regulated TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) transcription factors promote flowering in the facultative long-day Arabidopsis thaliana, but suppress it in the day-neutral tomato (Solanum lycopersicum). We combined genetic and molecular studies and described a new interplay between GA and two unrelated miRNA-associated pathways that modulates tomato transition to flowering. Tomato PROCERA/DELLA activity is required to promote flowering along with the miR156-targeted SQUAMOSA PROMOTER BINDING-LIKE (SPL/SBP) transcription factors by activating SINGLE FLOWER TRUSS (SFT) in the leaves and the MADS-Boxgene APETALA1(AP1)/MC at the shoot apex. Conversely, miR319-targeted LANCEOLATE represses floral transition by increasing GA concentrations and inactivating SFT in the leaves and AP1/MC at the shoot apex. Importantly, the combination of high GA concentrations/responses with the loss of SPL/SPB function impaired canonical meristem maturation and flower initiation in tomato. Our results reveal a cooperative regulation of tomato floral induction and flower development, integrating age cues (miR156 module) with GA responses and miR319-controlled pathways. Importantly, this study contributes to elucidate the mechanisms underlying the effects of GA in controlling flowering time in a day-neutral species. We thank Dr C. Schommer for kindly providing tcp4-soj8/+ seeds, and Carlos Rojas for Arabidopsis flowering time analyses. This work was supported by FAPESP (grant no. 15/17892-7 and fellowships nos 15/23826-7 and 13/16949-0). The authors declare no conflict of interest. |
| Databáze: | OpenAIRE |
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