Urinary N-Acetyl-Beta-D-Glucosaminidase Activity in Rat Experimental Ischemic and Toxic Models of Acute Kidney Injury
| Autor: | Sidonia Bogdan, Vlad Luca, Alexandra Biris, Ionut Ionut, Ileana Bogdan, Andras Nagy, Liviu Oana, Mircea Mircean, Aurora Livia Ursache, Razvan Codea, Orsolya Sarpataki, Cistian Popovici |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
lcsh:Veterinary medicine business.industry urogenital system Urinary system Acute kidney injury Pharmaceutical Science N-Acetyl-β-D-Glucosaminidase NAG acute kidney injury ischemia/reperfusion gentamicin nephrotoxicity medicine.disease urologic and male genital diseases Endocrinology Complementary and alternative medicine Internal medicine N-acetyl-beta-D-glucosaminidase Medicine lcsh:SF600-1100 Pharmacology (medical) business |
| Zdroj: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine, Vol 74, Iss 1, Pp 105-110 (2017) |
| ISSN: | 1843-5378 1843-5270 |
| Popis: | The identification of a suitable prevention method which facilitates limiting the deleterious effects of acute kidney injuries is highly required. In order to identify a proper treatment for acute kidney injuries, a suitable experimental model that replicates the structural, metabolic and inflammatory lesions that occur in the natural acute injured kidney is highly necessary. Intense urinary NAG activity can be found in a variety of renal disease such as toxic nephropathies, ischemic renal injury following cardiac surgery or renal transplantation but also in glomerular disease especially in diabetic nephropathy. Rises in urinary NAG enzyme activity strongly suggests tubular cell damage and support NAG enzyme as a biomarker of renal tubular injury. The aim of this paper is to obtain a stable in vivo acute kidney injury experimental model, in Wistar, rats and to evaluate the urinary activity of N-acetyl-β-D-glucosaminidase (NAG) enzyme, blood levels of urea and creatinine and microstructural renal alterations induced by ischemia/reperfusion injury respectively gentamicin nephrotoxicity. For this purpose we have used a rat experimental model. Adult male Wistar rats weighing 250-300 g were randomly divided into 3 groups with 8 rats in each group. Group 1 served as a model for the renal ischemia/reperfusion injury experiment, group 2 served for toxic kidney injury experimental model and group 3 served as control group. All individuals in both groups 1 and 2 presented marked elevations in blood urea and creatinine at the moment of euthanasia (day 3 for group 1 and day 9 for group 2) compared to the control group where biochemical values remained within normal limits. Urine analysis of both group 1 and 2 showed marked urinary NAG index activity which suggests acute tubular injury, suggestion confirmed by histological evaluation of the renal parenchyma sampled from this subjects |
| Databáze: | OpenAIRE |
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