Cytotoxic and mutagenic evaluation of extracts from plant species of the Miconia genus and their influence on doxorubicin-induced mutagenicity: an in vitro analysis
| Autor: | Eliana Aparecida Varanda, Ilce Mara de Syllos Cólus, Karina Yanagui, Gustavo Rafael Mazzaron Barcelos, Wagner Vilegas, Mateus Prates Mori, Juliana Mara Serpeloni |
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| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
Mutagen Biology Toxicology medicine.disease_cause Pathology and Forensic Medicine Cell Line Cricetulus Cricetinae Botany medicine Animals Clonogenic assay Cytotoxicity Micronuclei Chromosome-Defective Micronucleus Tests Traditional medicine Dose-Response Relationship Drug Plant Extracts Antimutagenic Agents Cell Biology General Medicine Fibroblasts Plant Components Aerial biology.organism_classification Doxorubicin Miconia Micronucleus test Melastomataceae Micronucleus Antimutagen Genotoxicity DNA Damage Mutagens |
| Zdroj: | Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. 63(5) |
| ISSN: | 1618-1433 |
| Popis: | The Miconia genus, a plant widely used for medicine, occurs in tropical America and its extracts and isolated compounds have demonstrated antibiotic, antitumoral, analgesic and antimalarial activities. However, no study concerning its genotoxicity has been conducted and it is necessary to determine its potential mutagenic effects to develop products and chemicals from these extracts. This study assessed the cytotoxicity, mutagenicity and the protective effects of methanolic extracts from Miconia species on Chinese hamster lung fibroblast cell cultures (V79). The cytotoxicity was evaluated using a clonogenic assay. Cultures exposed to the extract of Miconia albicans up to a concentration of 30 μg/mL, M. cabucu up to 40 μg/mL, M. albicans up to 40 μg/mL and M. stenostachya up to 60 μg/mL exhibited a cytotoxic effect on the cells. The clonogenic assay used three non-cytotoxic concentrations (5, 10 and 20 μg/mL) to evaluate mutagenicity and antimutagenicity of the extracts. Cultures were treated with these three extract concentrations (mutagenicity test) or the extract associated with doxorubicin (DXR) (antimutagenicity test) in three protocols (pre-, simultaneous and post-treatments). Distilled water and DXR were used as negative and positive controls, respectively. In the micronucleus (MN) test, a significant reduction was observed in MN frequency in cultures treated with DXR and extracts compared to those receiving only DXR; a significant reduction was also observed for the presence of mutagenicity in all treatments. This study confirmed the safe use of Miconia extracts at the concentrations tested and reinforced the therapeutic properties previously described for Miconia species by showing their protective effects on doxorubicin-induced mutagenicity. |
| Databáze: | OpenAIRE |
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