Mucosal Monosaccharide Transporter Expression in Newborns With Jejunoileal Atresia and Along the Adult Intestine
| Autor: | Simone M. R. Camargo, Stefan Holland-Cunz, C. Meier, Schirin Hunziker, Svenja Leu, François Verrey, Raphael N. Vuille-dit-Bille |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Monosaccharide Transport Proteins Intestinal Atresia Ileum 03 medical and health sciences 0302 clinical medicine Sodium-Glucose Transporter 1 030225 pediatrics Internal medicine Intestine Small medicine Humans RNA Messenger Aged Glucose Transporter Type 2 Mucous Membrane biology business.industry Glucose Transporter Type 5 Intestinal atresia Gastroenterology Glucose transporter Infant Newborn Middle Aged medicine.disease medicine.anatomical_structure Endocrinology Jejunum Atresia Pediatrics Perinatology and Child Health biology.protein Duodenum GLUT2 030211 gastroenterology & hepatology GLUT1 Female business GLUT5 |
| Zdroj: | Journal of pediatric gastroenterology and nutrition. 69(5) |
| ISSN: | 1536-4801 |
| Popis: | Objectives In newborn rodents, intestinal maturation involves delayed fructose transporter GLUT5 expression until weaning. In jejunoileal atresia (JIA), distal intestinal segments lack exposure to amniotic fluid-containing carbohydrates. We assessed in human newborns, the impact of intestinal maturation and obstruction on mucosal monosaccharide transporter expression. Methods Samples were obtained from 10 newborns operated for small intestinal atresia and from 17 adults undergoing gastroduodenoscopy and/or ileocolonoscopy. mRNA expression of the transporters SGLT1, GLUT1, GLUT2, GLUT5, and GLUT7 was measured in neonate samples proximal and distal of the atresia as well as in adult duodenum, ileum, and colon. Protein expression and localization was assessed using immunofluorescence. Results Although mRNA expression of monosaccharide transporters did not significantly differ between newborn and adult samples, luminal fructose transporter GLUT5 protein was absent in 0- to 4-day-old neonates, but expressed in adults. The mRNA expression of the 5 tested monosaccharide transporters was unchanged distal from the JIA relative to proximal. Similarly, luminal sodium-dependent glucose transporter SGLT1 and basolateral GLUT2 were expressed proximal and distal to JIA as visualized by immunofluorescence staining. With the exception of glucose transporter GLUT1 that showed highest expression levels in colon, all investigated hexose transporters showed strongest expression in duodenum, lower levels in ileum and lowest in colon. Conclusions Human newborns lack small intestinal fructose transporter GLUT5 protein expression and small intestinal atresia does not affect the expression of hexose transporters. |
| Databáze: | OpenAIRE |
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