Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China

Autor:	Dacheng Yan, Lijing Wu, Chunpu Li, Lihua Zhang, Tingfu Wang, Dan Liu, Minzhi Cao, Gaowa, Rongxin Liu, Shuji Ando, Ting Gao, Saixia Lu, Hong Li, Zitong Zhang, Xuhong Yin, Kozue Sato, Yunhong Cui, Guodong Wang, Hiroki Kawabata, Norio Ohashi, Wulantuya
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine MLSA China relapsing fever 030231 tropical medicine Borrelia miyamotoi Ixodes persulcatus Infectious and parasitic diseases RC109-216 Tick Real-Time Polymerase Chain Reaction law.invention 03 medical and health sciences 0302 clinical medicine Lyme disease law Borrelia medicine Animals Humans Polymerase chain reaction Ixodes biology Research Relapsing Fever Genomics biology.organism_classification medicine.disease bacterial infections and mycoses Virology Bacterial Typing Techniques Inner Mongolia 030104 developmental biology Infectious Diseases Parasitology Epidemiological Monitoring Multilocus Sequence Typing
Zdroj:	Parasites & Vectors, Vol 14, Iss 1, Pp 1-8 (2021) Parasites & Vectors
ISSN:	1756-3305
Popis:	Background Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus ticks, and we aimed to isolateB. miyamotoi from I. persulcatus from four regions of Greater Khingan, Inner Mongolia, China. Methods From May to June each year during the period 2016–2019, host-seeking adult I. persulcatus ticks were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate B. miyamotoi in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect Borrelia DNA in the ticks and to calculate the prevalence of B. miyamotoi and infections with other borreliae. For characterization of the isolated B. miyamotoi, we performed draft genome sequencing and multilocus sequencing analysis (MLSA). Results A total of 2656 adult I. persulcatus ticks were collected. The overall prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134/2656) and that of Lyme disease (LD) borreliae was 43.8% (1164/2656). Co-infection with RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae by qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (glpQ) PCR amplification and sequencing, through which we identified the RF borrelia specimens as B. miyamotoi. Furthermore, the B. miyamotoi strain Hetao-1 was isolated from I. persulcatus, and a draft genome sequence was obtained from the isolate. Sequencing determined the strain Hetao-1 genome to be approximately 906.1 kbp in length (28.9% average GC content), and MLSA identified the strain as ST633, which has previously been reported in Japan and Mongolia. Conclusion We detected B. miyamotoi from I. persulcatus ticks collected in Inner Mongolia, and successfully isolated a B. miyamotoi strain. To our knowledge, this is the first study to culture a B. miyamotoi isolate from China. The data on the prevalence of B. miyamotoi and other borreliae in I. persulcatus ticks will be fundamental for future epidemiological studies of B. miyamotoi disease in Inner Mongolia.
Databáze:	OpenAIRE
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